ArticleFrontiers in cell and developmental biology2021
New Insights Into Sperm Ultrastructure Through Enhanced Scanning Electron Microscopy.
Article in Frontiers in cell and developmental biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 18 citations in OpenAlex.
- Could glyoxal be an alternative fixative to paraformaldehyde in human spermatozoa?Journal of assisted reproduction and genetics · 2026Article
- Effects of Diet-Induced Obesity and Exercise Intervention on Testicular Germ Cell Health: Modulation of SIRT1/FoxO1 and SIRT1/PGC-1α Signaling Axes.Cell biochemistry and biophysics · 2026Article
- Germ cell risk assessment of combined exposure to monosodium glutamate and bisphenol A in male SD rat: deciphering the molecular mechanisms.Naunyn-Schmiedeberg's archives of pharmacology · 2026Article
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- Uncovering candidate protein(s) in murine vaginal lavage fluid linked to sperm-agglutinating Escherichia coli-induced infertility menace: a discovery phase analysis.Archives of microbiology · 2026Article
- In vivo semen characterization and seasonal variation in Procavia capensis.Reproduction & fertility · 2026Article
- CCDC89 is required for optimal sperm motility and male fertility in mammals.Human reproduction (Oxford, England) · 2025Article
- A novel mechanism of sperm midpiece epididymal maturation and the role of CCDC112 in sperm midpiece formation and establishing an optimal flagella waveform.Cell communication and signaling : CCS · 2025Article
- Impact of air pollution and heavy metal exposure on sperm quality: A clinical prospective research study.Toxicology reports · 2024Article
- Critical role of Spatio-Temporally Regulated Maternal RNAs in Zebrafish Embryogenesis.bioRxiv : the preprint server for biology · 2024Article
- AXDND1 is required to balance spermatogonial commitment and for sperm tail formation in mice and humans.Cell death & disease · 2024Article
- Genetic mutation ofLife science alliance · 2024Article
- The Cby3/ciBAR1 complex positions the annulus along the sperm flagellum during spermiogenesis.The Journal of cell biology · 2024Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The analysis of spermatozoa morphology is fundamental to understand male fertility and the etiology of infertility. Traditionally scanning electron microscopy (SEM) has been used to define surface topology. Recently, however, it has become a critical tool for three-dimensional analysis of internal cellular ultrastructure. Modern SEM provides nanometer-scale resolution, but the meaningfulness of such information is proportional to the quality of the sample preservation. In this study, we demonstrate that sperm quickly and robustly adhere to gold-coated surfaces. Leveraging this property, we developed three step-by-step protocols fulfilling different needs for sperm imaging: chemically fixed monolayers for SEM examination of the external morphology, and two high-pressure freezing-based protocols for fast SEM examination of full cell internal morphology and focused ion-beam SEM tomography. These analyses allow previously unappreciated insights into mouse sperm ultrastructure, including the identification of novel structures within the fibrous sheath and domain-specific interactions between the plasma membrane and exosome-like structures.
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