Evidence map›Paper›PMID 33968935›Full record

ArticleFrontiers in cell and developmental biology2021

Fluorescence-Based Ratiometric Analysis of Sperm Centrioles (FRAC) Finds Patient Age and Sperm Morphology Are Associated With Centriole Quality.

Katerina A Turner, Emily L Fishman, Mariam Asadullah, Brooke Ott, Patrick Dusza, Tariq A Shah, Puneet Sindhwani, Nagalakshmi Nadiminty, Emanuela Molinari, Pasquale Patrizio and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in cell and developmental biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
2.7field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 19 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

Katerina A TurnerDepartment of Biological Sciences, College of Natural Sciences and Mathematics, University of Toledo, Toledo, OH, United States.
Emily L FishmanDepartment of Biological Sciences, College of Natural Sciences and Mathematics, University of Toledo, Toledo, OH, United States.
Mariam AsadullahDepartment of Biological Sciences, College of Natural Sciences and Mathematics, University of Toledo, Toledo, OH, United States.
Brooke OttDepartment of Biological Sciences, College of Natural Sciences and Mathematics, University of Toledo, Toledo, OH, United States.
Patrick DuszaDepartment of Biological Sciences, College of Natural Sciences and Mathematics, University of Toledo, Toledo, OH, United States.
Tariq A ShahDepartment of Urology, College of Medicine and Life Sciences, University of Toledo, Toledo, OH, United States.
Puneet SindhwaniDepartment of Urology, College of Medicine and Life Sciences, University of Toledo, Toledo, OH, United States.
Nagalakshmi NadimintyDepartment of Urology, College of Medicine and Life Sciences, University of Toledo, Toledo, OH, United States.
Emanuela MolinariYale Fertility Center, Yale School of Medicine, New Haven, CT, United States.
Pasquale PatrizioYale Fertility Center, Yale School of Medicine, New Haven, CT, United States.
Barbara S SaltzmanSchool of Population Health, College of Health and Human Services, University of Toledo, Toledo, OH, United States.
Tomer Avidor-ReissDepartment of Biological Sciences, College of Natural Sciences and Mathematics, University of Toledo, Toledo, OH, United States.
University of Toledo · USYale Cancer Center · US

Funding

Molecular Marker for Centriole Remodeling in Human ReproductionR03HD098314 · NICHD · UNIVERSITY OF TOLEDO · PI AVIDOR-REISS, TOMER · 2019 to 2020
$150k
NICHD NIH HHS R03 HD098314
6 · The paper itself

Abstract

A large proportion of infertility and miscarriage causes are unknown. One potential cause is a defective sperm centriole, a subcellular structure essential for sperm motility and embryonic development. Yet, the extent to which centriolar maladies contribute to male infertility is unknown due to the lack of a convenient way to assess centriole quality. We developed a robust, location-based, ratiometric assay to overcome this roadblock, the Fluorescence-based Ratiometric Assessment of Centrioles (FRAC). We performed a case series study with semen samples from 33 patients, separated using differential gradient centrifugation into higher-grade (pellet) and lower-grade (interface) sperm fractions. Using a reference population of higher-grade sperm from infertile men with morphologically standard sperm, we found that 79% of higher-grade sperm of infertile men with substandard sperm morphology have suboptimal centrioles (

Indexed as

agecentriolefluorescenceinfertilitymethodmiscarriagespermteratospermia

Identifiers

PMID33968935
PMCPMC8100587
OpenAlexW3161984644

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.