ArticleFrontiers in microbiology2021
Rapid and Sensitive Detection of Tomato Brown Rugose Fruit Virus in Tomato and Pepper Seeds by Reverse Transcription Loop-Mediated Isothermal Amplification Assays (Real Time and Visual) and Comparison With RT-PCR End-Point and RT-qPCR Methods.
Article in Frontiers in microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 48 citations in OpenAlex.
- Tobamoviruses: Advances in Molecular Biology, Host Interactions and Integrated Disease Management.Biology · 2026Review
- Comprehensive high-throughput sequence-based detection and RT-LAMP confirmation of RNA viruses associated with dragon fruit (Selenicereus spp.) in India.Archives of virology · 2026Article
- Effect of low-dose gamma irradiation on seed-borne transmission of tomato brown rugose fruit virus in tomato.Journal, genetic engineering & biotechnology · 2026Article
- Detection of tomato brown rugose fruit virus through CRISPR-Cas12a and CRISPR-Cas9 systems.Scientific reports · 2025Article
- Advanced molecular tools for surveillance and management of tobamoviruses.Frontiers in plant science · 2025Review
- Genomic insights into host-associated variants and transmission features of a ToBRFV isolate from Mexico.Frontiers in plant science · 2025Article
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- A reverse transcription loop-mediated isothermal amplification assay for quick detection of tomato mosaic virus.PloS one · 2024Article
- Revealing an Iranian Isolate of Tomato Brown Rugose Fruit Virus: Complete Genome Analysis and Mechanical Transmission.Microorganisms · 2023Article
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- Pepper Plants HarboringPlants (Basel, Switzerland) · 2022Article
- Tomato brown rugose fruit virus: An emerging and rapidly spreading plant RNA virus that threatens tomato production worldwide.Molecular plant pathology · 2022Review
- Viruses of Economic Impact on Tomato Crops in Mexico: From Diagnosis to Management-A Review.Viruses · 2022Review
- The Potential Use of Isothermal Amplification Assays for In-Field Diagnostics of Plant Pathogens.Plants (Basel, Switzerland) · 2021Review
- New Resources for the Specific and Sensitive Detection of the Emerging Tomato Brown Rugose Fruit Virus.Viruses · 2021Article
- Differential Detection of the Tobamoviruses Tomato Mosaic Virus (ToMV) and Tomato Brown Rugose Fruit Virus (ToBRFV) Using CRISPR-Cas12a.Plants (Basel, Switzerland) · 2021Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tomato brown rugose fruit virus (ToBRFV) represents an emerging viral threat to the productivity of tomato and pepper protected cultivation worldwide. This virus has got the status of quarantine organism in the European Union (EU) countries. In particular, tomato and pepper seeds will need to be free of ToBRFV before entering the EU and before coming on the market. Thus, lab tests are needed. Here, we develop and validate a one-step reverse transcription LAMP platform for the detection of ToBRFV in tomato and pepper leaves, by real-time assay [reverse transcription loop-mediated isothermal amplification (RT-LAMP)] and visual screening (visual RT-LAMP). Moreover, these methods can also be applied successfully for ToBRFV detection in tomato and pepper seeds. The diagnostic specificity and sensitivity of both RT-LAMP and visual RT-LAMP are both 100%, with a detection limit of nearly 2.25 fg/μl, showing the same sensitivity as RT-qPCR Sybr Green, but 100 times more sensitive than end-point RT-PCR diagnostic methods. In artificially contaminated seeds, the proposed LAMP assays detected ToBRFV in 100% of contaminated seed lots, for up to 0.025-0.033% contamination rates in tomato and pepper, respectively. Our results demonstrate that the proposed LAMP assays are simple, inexpensive, and sensitive enough for the detection of ToBRFV, especially in seed health testing. Hence, these methods have great potential application in the routine detection of ToBRFV, both in seeds and plants, reducing the risk of epidemics.
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