Evidence map›Paper›PMID 33965839›Full record

ArticleBioorganic & medicinal chemistry2021

Spectroscopic and biochemical characterization of metallo-β-lactamase IMP-1 with dicarboxylic, sulfonyl, and thiol inhibitors.

Huan Zhang, Kundi Yang, Zishuo Cheng, Caitlyn Thomas, Abbie Steinbrunner, Cecily Pryor, Maya Vulcan, Claire Kemp, Diego Orea, Chathura Paththamperuma and 5 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioorganic & medicinal chemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.4field-weighted citation impact, top 48% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Machine Learning Models Identify Inhibitors of New Delhi Metallo-β-lactamase.Journal of chemical information and modeling · 2024
    Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 2 institutions in 1 country.

Huan ZhangDepartment of Chemistry and Biochemistry, Miami University, Oxford, OH 45056, USA.
Kundi YangDepartment of Chemistry and Biochemistry, Miami University, Oxford, OH 45056, USA.
Zishuo ChengDepartment of Chemistry and Biochemistry, Miami University, Oxford, OH 45056, USA.
Caitlyn ThomasDepartment of Chemistry and Biochemistry, Miami University, Oxford, OH 45056, USA.
Abbie SteinbrunnerDepartment of Chemistry and Biochemistry, Miami University, Oxford, OH 45056, USA.
Cecily PryorDepartment of Chemistry and Biochemistry, Miami University, Oxford, OH 45056, USA.
Maya VulcanDepartment of Chemistry and Biochemistry, Miami University, Oxford, OH 45056, USA.
Claire KempDepartment of Chemistry and Biochemistry, Miami University, Oxford, OH 45056, USA.
Diego OreaDepartment of Chemistry and Biochemistry, Miami University, Oxford, OH 45056, USA.
Chathura PaththamperumaDepartment of Chemistry and Biochemistry, Miami University, Oxford, OH 45056, USA.
Allie Y ChenDepartment of Chemistry and Biochemistry, University of California San Diego, La Jolla, CA 92093, USA.
Seth M CohenDepartment of Chemistry and Biochemistry, University of California San Diego, La Jolla, CA 92093, USA.
Richard C PageDepartment of Chemistry and Biochemistry, Miami University, Oxford, OH 45056, USA.
David L TierneyDepartment of Chemistry and Biochemistry, Miami University, Oxford, OH 45056, USA.
Michael W CrowderDepartment of Chemistry and Biochemistry, Miami University, Oxford, OH 45056, USA. Electronic address: crowdemw@miamioh.edu.
Miami University · USUniversity of California San Diego · US

Funding

Triage mechanisms for directing protein refolding and degradationR35GM128595 · NIGMS · MIAMI UNIVERSITY OXFORD · PI Richard C Page · 2018 to 2026
$2.8M
Developing Metallo-Beta-Lactamase InhibitorsR01GM111926 · NIGMS · UNIVERSITY OF TEXAS AT AUSTIN · PI BONOMO, ROBERT A., COHEN, SETH M · 2015 to 2019
$2.3M
Metal-binding Isosteres for Influenza Endonuclease Inhibitors and BeyondR01AI149444 · NIAID · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI SETH M COHEN · 2020 to 2026
$2.3M
Machine Learning Approach for finding novel metallo-b-lactamase inhibitorsR15GM134454 · NIGMS · MIAMI UNIVERSITY OXFORD · PI CROWDER, MICHAEL W · 2019 to 2022
$867k
NIAID NIH HHS R01 AI149444NIGMS NIH HHS R01 GM111926NIGMS NIH HHS R15 GM134454NIGMS NIH HHS R35 GM128595
6 · The paper itself

Abstract

In an effort to probe the biophysical mechanisms of inhibition for ten previously-reported inhibitors of metallo-β-lactamases (MBL) with MBL IMP-1, equilibrium dialysis, metal analyses coupled with atomic absorption spectroscopy (AAS), native state mass spectrometry (native MS), and ultraviolet-visible spectrophotometry (UV-VIS) were used. 6-(1H-tetrazol-5-yl) picolinic acid (1T5PA), ANT431, D/l-captopril, thiorphan, and tiopronin were shown to form IMP-1/Zn(II)/inhibitor ternary complexes, while dipicolinic acid (DPA) and 4-(3-aminophenyl)pyridine-2,6-dicarboxylic acid (3AP-DPA) stripped some metal from the active site of IMP but also formed ternary complexes. DPA and 3AP-DPA stripped less metal from IMP-1 than from VIM-2 but stripped more metal from IMP-1 than from NDM-1. In contrast to a previous report, pterostilbene does not appear to bind to IMP-1 under our conditions. These results, along with previous studies, demonstrate similar mechanisms of inhibition toward different MBLs for different MBL inhibitors.

Indexed as

beta-LactamasesDicarboxylic AcidsDose-Response Relationship, DrugEnzyme InhibitorsMass SpectrometryMolecular StructurePseudomonas aeruginosaSerratia marcescensSpectrophotometry, AtomicSpectrophotometry, UltravioletStructure-Activity RelationshipSulfhydryl CompoundsSulfidesbeta-lactamase IMP-1beta-LactamasesDicarboxylic AcidsEnzyme InhibitorsSulfhydryl CompoundsSulfidesAntibiotic resistanceIMP-1MBL inhibitorsMetallo-β-lactamaseNative state mass spectrometry

Identifiers

PMID33965839
PMCPMC8170513
OpenAlexW3159019910

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.