ArticleMicrobial cell factories2021
Genetic engineering of the precursor supply pathway for the overproduction of the nC
Article in Microbial cell factories, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 33 citations in OpenAlex.
- Genomic and functional characterization of Bacillus subtilis subsp. subtilis INPA R296 with emulsifying activity in cultivation with residual soybean oil.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- Lipopeptide Engineering: From Natural Origins to Rational Design Against Antimicrobial Resistance.Antibiotics (Basel, Switzerland) · 2026Review
- Genomic and transcriptomic analysis of yield enhancement mechanism of lipopeptide iturin W by tryptone supplementation.Microbiology spectrum · 2026Article
- Article
- Regulation mechanism and bioactivity characteristic of surfactin homologues with C14 and C15 fatty acid chains.Microbial cell factories · 2024Article
- Biosurfactant and biopolymer producing microorganisms from West Kazakhstan oilfield.Scientific reports · 2024Article
- Exploring the use of microbial enhanced oil recovery in Kazakhstan: a review.Frontiers in microbiology · 2024Review
- Antimicrobial peptides from Bacillus spp. and strategies to enhance their yield.Applied microbiology and biotechnology · 2023Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundSurfactin, a representative biosurfactant of lipopeptide mainly produced by Bacillus subtilis, consists of a cyclic heptapeptide linked to a β-hydroxy fatty acid chain. The functional activity of surfactin is closely related to the length and isomerism of the fatty acid chain.
resultsIn this study, the fatty acid precursor supply pathway in Bacillus subtilis 168 for surfactin production was strengthened through two steps. Firstly, pathways competing for the precursors were eliminated with inactivation of pps and pks. Secondly, the plant medium-chain acyl-carrier protein (ACP) thioesterase (BTE) from Umbellularia californica was overexpressed. As a result, the surfactin titer after 24 h of cultivation improved by 34%, and the production rate increased from 0.112 to 0.177 g/L/h. The isoforms identified by RP-HPLC and GC-MS showed that the proportion of nC
conclusionThe obtained results identified that enhancing the supply of fatty acid precursor is very essential for the synthesis of surfactin. At the same time, this study also proved that thioesterase BTE can promote the production of nC
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.