ReviewNature methods2021
Understanding the invisible hands of sample preparation for cryo-EM.
Review in Nature methods, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 101 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
101 citing papers in PubMed, 184 citations in OpenAlex.
- A pseudo-labeling approach for automated assignment of unlabeled proteins exceeding 100 amino acid residues.Magnetic resonance letters · 2027Article
- Acoustofluidic cryo-EM enables in situ particle manipulation for uniform, high-quality cryo-EM specimens.Science advances · 2026Article
- Control of ice thickness in cryo-EM via confinement.Nature methods · 2026Article
- Cryo-EM sample preparation with soft-landing and laser flash melting.Nature communications · 2026Article
- Direct deposition on pre-cooled grids: a new approach to sub-millisecond cryoEM grid preparation.Acta crystallographica. Section D, Structural biology · 2026Article
- EasyGrid: a versatile platform for automated cryo-EM sample preparation and quality control.Nature methods · 2026Article
- Incorporating Surfaced-Induced Dissociation Mass Spectrometry Data into an AlphaFold-derived deep learning network improves protein structure prediction.bioRxiv : the preprint server for biology · 2026Article
- prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.Structure (London, England : 1993) · 2026Article
- A cost-effective 200 kV cryo-EM core facility for high resolution single particle analysis.Methods in microscopy · 2026Article
- Purifying recombinant proteins from Nicotiana benthamiana for structural studies.Nature protocols · 2026Review
- An efficient clear-native PAGE-based workflow for cryo-electron microscopy sample preparation of large protein complexes.Plant methods · 2026Article
- Breaking barriers: transitioning from X-ray crystallography to cryo-EM for structural studies.Acta crystallographica. Section D, Structural biology · 2026Article
- PARP1-HPF1 structure and dynamics on nicked DNA suggest a mechanism for acute and localized ADP-ribosylation.Nature communications · 2026Article
- Cryogenic transmission electron microscopy reveals assembly and nanostructure of PEDOT:PSS.Nature communications · 2026Article
- Microfluidic Devices for High-Throughput In Situ Serial Crystallography.Advances in biochemical engineering/biotechnology · 2026Review
- Isolation and Visualization Fusobacterium nucleatum Microcompartments.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Systematic scale-up and enhanced purification of marine cyanophage P-SSP7.Frontiers in microbiology · 2026Article
- Nickel-NTA lipid-monolayer affinity grids allow for high-resolution structure determination by cryo-EM.Journal of structural biology · 2025Article
- Nanoscale Imaging of Biological Tissues: Techniques, Challenges and Emerging Frontiers.Nanomaterials (Basel, Switzerland) · 2025Review
- Article
41 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cryo-electron microscopy (cryo-EM) is rapidly becoming an attractive method in the field of structural biology. With the exploding popularity of cryo-EM, sample preparation must evolve to prevent congestion in the workflow. The dire need for improved microscopy samples has led to a diversification of methods. This Review aims to categorize and explain the principles behind various techniques in the preparation of vitrified samples for the electron microscope. Various aspects and challenges in the workflow are discussed, from sample optimization and carriers to deposition and vitrification. Reliable and versatile specimen preparation remains a challenge, and we hope to give guidelines and posit future directions for improvement.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.