ArticleApplied and environmental microbiology2021
Engineering Posttranslational Regulation of Glutamine Synthetase for Controllable Ammonia Production in the Plant Symbiont Azospirillum brasilense.
Article in Applied and environmental microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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Who cites it
21 citing papers in PubMed, 28 citations in OpenAlex.
- Challenges and Future Directives of Synthetic Biology in Engineering Plant-Microbe Partnerships for Sustainable Agriculture.Biotechnology and bioengineering · 2026Review
- PGPR inoculation and growth enhancement of crops cultivated in hydroponic systems.World journal of microbiology & biotechnology · 2026Review
- Engineered diazotrophs with host-inducible nitrogen supply systems: Transforming rice farming through innovative nitrogen biofertilizers.Journal of integrative plant biology · 2026Review
- Developing improved inoculants through genome editing of diazotrophic bacteria for cereals: Is this the right time for Brazil?Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Industrial Microbial Technologies for Feed Protein Production from Non-Protein Nitrogen.Microorganisms · 2025Review
- Synthetic communities as a model for determining interactions between a biofertilizer chassis organism and native microbial consortia.The ISME journal · 2025Article
- Inoculation and co-inoculation of lettuce and arugula hydroponically influence nitrogen metabolism, plant growth, nutrient acquisition and photosynthesis.Frontiers in plant science · 2025Article
- Competitive fitness and stability of ammonium-excreting Azotobacter vinelandii strains in the soil.Applied microbiology and biotechnology · 2024Article
- Scripting a new dialogue between diazotrophs and crops.Trends in microbiology · 2024Review
- Choreographing root architecture and rhizosphere interactions through synthetic biology.Nature communications · 2024Review
- Diurnal switches in diazotrophic lifestyle increase nitrogen contribution to cereals.Nature communications · 2023Article
- Multiplicity of theACS synthetic biology · 2023Article
- Microbiota-mediated nitrogen fixation and microhabitat homeostasis in aerial root-mucilage.Microbiome · 2023Article
- Rhizopine biosensors for plant-dependent control of bacterial gene expression.Environmental microbiology · 2023Article
- Molecular Mechanism and Agricultural Application of the NifA-NifL System for Nitrogen Fixation.International journal of molecular sciences · 2023Review
- Control of nitrogen fixation and ammonia excretion in Azorhizobium caulinodans.PLoS genetics · 2022Article
- Engineered plant control of associative nitrogen fixation.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Genetic Circuit Design in Rhizobacteria.Biodesign research · 2022Review
- Improved Stability of Engineered Ammonia Production in the Plant-SymbiontACS synthetic biology · 2021Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nitrogen requirements for modern agriculture far exceed the levels of bioavailable nitrogen in most arable soils. As a result, the addition of nitrogen fertilizer is necessary to sustain productivity and yields, especially for cereal crops, the planet's major calorie suppliers. Given the unsustainability of industrial fertilizer production and application, engineering biological nitrogen fixation directly at the roots of plants has been a grand challenge for biotechnology. Here, we designed and tested a potentially broadly applicable metabolic engineering strategy for the overproduction of ammonia in the diazotrophic symbiont Azospirillum brasilense. Our approach is based on an engineered unidirectional adenylyltransferase (uAT) that posttranslationally modifies and deactivates glutamine synthetase (GS), a key regulator of nitrogen metabolism in the cell. We show that this circuit can be controlled inducibly, and we leveraged the inherent self-contained nature of our posttranslational approach to demonstrate that multicopy redundancy can improve strain evolutionary stability. uAT-engineered
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.