ArticleBiology of reproduction2021
Microvesicles and exosomes released by amnion epithelial cells under oxidative stress cause inflammatory changes in uterine cells†.
Article in Biology of reproduction, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
30 citing papers in PubMed, 44 citations in OpenAlex.
- Molecular Profiling of Extracellular Vesicles Isolated from Boar Reproductive Fluids.Journal of extracellular vesicles · 2026Article
- Human amnion epithelial cells induce M2 macrophage polarisation partially via M-CSF secretion but independently of extracellular vesiclesFrontiers in immunology · 2026Article
- Role of Seminal Exosomes in Reproduction.Journal of cellular physiology · 2025Review
- Exofection by exosomes: A transient functional cargo transfer.Extracellular vesicle · 2025Article
- Exosomal delivery of IL-10: Biodistribution, pharmacokinetics, and preterm birth prevention strategies.Extracellular vesicle · 2025Article
- The Impact of the Delivery Method on Oxidative Stress in Neonates: A Cross-Sectional Study.Journal of clinical medicine · 2025Article
- Exploring the link between M1 macrophages and EMT of amniotic epithelial cells: implications for premature rupture of membranes.Journal of nanobiotechnology · 2025Article
- Oxidative Score and Microvesicle Profile Suggest Cardiovascular Risk in Chronic Kidney Disease.Antioxidants (Basel, Switzerland) · 2025Article
- The role of amniotic epithelial cells in preterm birth: mechanisms and clinical implications.Frontiers in cell and developmental biology · 2025Review
- Large extracellular vesicles from induced pluripotent stem cell-marrow stem cells enhance limb angiogenesis via ERK/MAPK.Nanomedicine (London, England) · 2024Article
- Seminar: Extracellular Vesicles as Mediators of Environmental Stress in Human Disease.Environmental health perspectives · 2023Article
- The exosome: a review of current therapeutic roles and capabilities in human reproduction.Drug delivery and translational research · 2023Review
- Extracellular Vesicles-mediated recombinant IL-10 protects against ascending infection-associated preterm birth by reducing fetal inflammatory response.Frontiers in immunology · 2023Article
- Osteoimmune Interactions and Therapeutic Potential of Macrophage-Derived Small Extracellular Vesicles in Bone-Related Diseases.International journal of nanomedicine · 2023Review
- Amplification of microbial DNA from bacterial extracellular vesicles from human placenta.Frontiers in microbiology · 2023Article
- Coenzyme Q10 Stimulate Reproductive Vatality.Drug design, development and therapy · 2023Review
- Review on new approach methods to gain insight into the feto-maternal interface physiology.Frontiers in medicine · 2023Review
- Proteomic and miRNA Profiles of Exosomes Derived from Myometrial Tissue in Laboring Women.International journal of molecular sciences · 2022Article
- Biomaterials and Extracellular Vesicle Delivery: Current Status, Applications and Challenges.Cells · 2022Review
- Epithelial to mesenchymal transition (EMT) of feto-maternal reproductive tissues generates inflammation: a detrimental factor for preterm birth.BMB reports · 2022Article
Corrections and comments
- Erratum issued
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
Extracellular vesicles play a crucial role in feto-maternal communication and provide an important paracrine signaling mechanism in pregnancy. We hypothesized that fetal cells-derived exosomes and microvesicles (MVs) under oxidative stress (OS) carry unique cargo and traffic through feto-maternal interface, which cause inflammation in uterine cells associated with parturition. Exosomes and MVs, from primary amnion epithelial cell (AEC) culture media under normal or OS-induced conditions, were isolated by optimized differential centrifugation method followed by characterization for size (nanoparticle tracking analyzer), shape (transmission electron microscopy), and protein markers (western blot and immunofluorescence). Cargo and canonical pathways were identified by mass spectroscopy and ingenuity pathway analysis. Myometrial, decidual, and cervical cells were treated with 1 × 107 control/OS-derived exosomes/MVs. Pro-inflammatory cytokines were measured using a Luminex assay. Statistical significance was determined by paired T-test (P < 0.05). AEC produced cup-shaped exosomes of 90-150 nm and circular MVs of 160-400 nm. CD9, heat shock protein 70, and Nanog were detected in exosomes, whereas OCT-4, human leukocyte antigen G, and calnexin were found in MVs. MVs, but not exosomes, were stained for phosphatidylserine. The protein profiles for control versus OS-derived exosomes and MVs were significantly different. Several inflammatory pathways related to OS were upregulated that were distinct between exosomes and MVs. Both OS-derived exosomes and MVs significantly increased pro-inflammatory cytokines (granulocyte-macrophage colony-stimulating factor, interleukin 6 (IL-6), and IL-8) in maternal cells compared with control (P < 0.05). Our findings suggest that fetal-derived exosomes and MVs under OS exhibited distinct characteristics and a synergistic inflammatory role in uterine cells associated with the initiation of parturition.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.