Evidence map›Paper›PMID 33961793›Full record

ArticleStem cell reports2021

Hematopoietic stem cells retain functional potential and molecular identity in hibernation cultures.

Caroline A Oedekoven, Miriam Belmonte, Daniel Bode, Fiona K Hamey, Mairi S Shepherd, James Lok Chi Che, Grace Boyd, Craig McDonald, Serena Belluschi, Evangelia Diamanti and 5 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
2.5field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 26 citations in OpenAlex.

  1. Article
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  9. Cellular senescence: Neither irreversible nor reversible.The Journal of experimental medicine · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 3 institutions in 1 country.

Caroline A OedekovenWellcome MRC Cambridge Stem Cell Institute, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK; Department of Haematology, University of Cambridge, Cambridge CB2 0XY, UK.
Miriam BelmonteWellcome MRC Cambridge Stem Cell Institute, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK; Department of Haematology, University of Cambridge, Cambridge CB2 0XY, UK.
Daniel BodeWellcome MRC Cambridge Stem Cell Institute, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK; Department of Haematology, University of Cambridge, Cambridge CB2 0XY, UK.
Fiona K HameyWellcome MRC Cambridge Stem Cell Institute, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK; Department of Haematology, University of Cambridge, Cambridge CB2 0XY, UK.
Mairi S ShepherdWellcome MRC Cambridge Stem Cell Institute, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK; Department of Haematology, University of Cambridge, Cambridge CB2 0XY, UK.
James Lok Chi CheWellcome MRC Cambridge Stem Cell Institute, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK; Department of Haematology, University of Cambridge, Cambridge CB2 0XY, UK.
Grace BoydYork Biomedical Research Institute, Department of Biology, University of York, York YO10 5DD, UK.
Craig McDonaldWellcome MRC Cambridge Stem Cell Institute, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK; Department of Haematology, University of Cambridge, Cambridge CB2 0XY, UK.
Serena BelluschiWellcome MRC Cambridge Stem Cell Institute, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK; Department of Haematology, University of Cambridge, Cambridge CB2 0XY, UK.
Evangelia DiamantiWellcome MRC Cambridge Stem Cell Institute, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK; Department of Haematology, University of Cambridge, Cambridge CB2 0XY, UK.
Hugo P BastosWellcome MRC Cambridge Stem Cell Institute, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK; Department of Haematology, University of Cambridge, Cambridge CB2 0XY, UK.
Katherine S BridgeYork Biomedical Research Institute, Department of Biology, University of York, York YO10 5DD, UK.
Berthold GöttgensWellcome MRC Cambridge Stem Cell Institute, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK; Department of Haematology, University of Cambridge, Cambridge CB2 0XY, UK.
Elisa LaurentiWellcome MRC Cambridge Stem Cell Institute, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK; Department of Haematology, University of Cambridge, Cambridge CB2 0XY, UK.
David G KentWellcome MRC Cambridge Stem Cell Institute, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK; Department of Haematology, University of Cambridge, Cambridge CB2 0XY, UK; York Biomedical Research Institute, Department of Biology, University of York, York YO10 5DD, UK. Electronic address: david.kent@york.ac.uk.
Wellcome/MRC Cambridge Stem Cell Institute · GBUniversity of Cambridge · GBUniversity of York · GB

Funding

Biotechnology and Biological Sciences Research Council BB/P002293/1Cancer Research UKDepartment of HealthMedical Research Council MC_PC_16040Medical Research Council MC_PC_17230Medical Research Council MR/M008975/1Medical Research Council MR/V005502/1Wellcome Trust 107630/Z/15/ZWellcome Trust 206328/Z/17/Z
6 · The paper itself

Abstract

Advances in the isolation and gene expression profiling of single hematopoietic stem cells (HSCs) have permitted in-depth resolution of their molecular program. However, long-term HSCs can only be isolated to near purity from adult mouse bone marrow, thereby precluding studies of their molecular program in different physiological states. Here, we describe a powerful 7-day HSC hibernation culture system that maintains HSCs as single cells in the absence of a physical niche. Single hibernating HSCs retain full functional potential compared with freshly isolated HSCs with respect to colony-forming capacity and transplantation into primary and secondary recipients. Comparison of hibernating HSC molecular profiles to their freshly isolated counterparts showed a striking degree of molecular similarity, further resolving the core molecular machinery of HSC self-renewal while also identifying key factors that are potentially dispensable for HSC function, including members of the AP1 complex (Jun, Fos, and Ncor2), Sult1a1 and Cish. Finally, we provide evidence that hibernating mouse HSCs can be transduced without compromising their self-renewal activity and demonstrate the applicability of hibernation cultures to human HSCs.

Indexed as

TranscriptomeAnimalsArylsulfotransferaseBone Marrow TransplantationCell Culture TechniquesCell CycleCell DifferentiationCells, CulturedCytokinesHematopoietic Stem CellsHibernationMiceMice, Inbred C57BLMultiprotein ComplexesSignaling Lymphocytic Activation Molecule Family Member 1Single-Cell AnalysisArylsulfotransferaseCytokinesMultiprotein ComplexesSignaling Lymphocytic Activation Molecule Family Member 1Slamf1 protein, mouseSult1a1 protein, mouseSuppressor of Cytokine Signaling ProteinsTranscription Factor AP-1cell cyclehematopoietic stem cellsquiescencesingle-cell assayssingle-cell RNA sequencingstem cell nichetransplantation

Identifiers

PMID33961793
PMCPMC8190576
OpenAlexW3159064086

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.