Evidence map›Paper›PMID 33961791›Full record

ArticleStem cell reports2021

Neuroglian regulates Drosophila intestinal stem cell proliferation through enhanced signaling via the epidermal growth factor receptor.

Martin Resnik-Docampo, Kathleen M Cunningham, S Mateo Ruvalcaba, Charles Choi, Vivien Sauer, D Leanne Jones

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
0.8field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 11 citations in OpenAlex.

  1. Intrinsic regulation of intestinal stem cell fate and homeostasis by Tet.bioRxiv : the preprint server for biology · 2026
    Article
  2. Article
  3. International journal of molecular sciences · 2025
    Article
  4. Review
  5. Article
  6. Article
  7. Article
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Martin Resnik-DocampoDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Kathleen M CunninghamDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, USA.
S Mateo RuvalcabaDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Charles ChoiDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Vivien SauerDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, USA.
D Leanne JonesDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, USA; Molecular Biology Institute, University of California, Los Angeles, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA 90095, USA. Electronic address: leanne.jones@ucsf.edu.
University of California, Los Angeles · US

Funding

TUMOR CELL SURFACES AND CHROMOSOMEST32CA009056 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Gay M Crooks, David A. Nathanson · 1985 to 2026
$10.5M
Characterization of Age-Related Changes in Stem Cell BehaviorR01AG028092 · NIA · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI JONES, DANA LEANNE · 2007 to 2016
$3.6M
Lipid mediated regulation of stem cell behavior and tissue homeostasisR01GM135767 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI JONES, DANA LEANNE · 2020 to 2023
$1.8M
Regulation of intestinal stem cell behavior by occluding junctionsR01DK105442 · NIDDK · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI JONES, DANA LEANNE · 2017 to 2020
$1.7M
Regulation of intestinal tissue homeostasis by the transcription factor EscargotF32GM119394 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI CHOI, HYUN CHUL · 2016 to 2017
$111k
NCI NIH HHS T32 CA009056NIA NIH HHS R01 AG028092NIDDK NIH HHS R01 DK105442NIGMS NIH HHS F32 GM119394NIGMS NIH HHS R01 GM135767
6 · The paper itself

Abstract

The Drosophila intestine is an excellent system for elucidating mechanisms regulating stem cell behavior. Here we show that the septate junction (SJ) protein Neuroglian (Nrg) is expressed in intestinal stem cells (ISCs) and enteroblasts (EBs) within the fly intestine. SJs are not present between ISCs and EBs, suggesting Nrg plays a different role in this tissue. We reveal that Nrg is required for ISC proliferation in young flies, and depletion of Nrg from ISCs and EBs suppresses increased ISC proliferation in aged flies. Conversely, overexpression of Nrg in ISC and EBs promotes ISC proliferation, leading to an increase in cells expressing ISC/EB markers; in addition, we observe an increase in epidermal growth factor receptor (Egfr) activation. Genetic epistasis experiments reveal that Nrg acts upstream of Egfr to regulate ISC proliferation. As Nrg function is highly conserved in mammalian systems, our work characterizing the role of Nrg in the intestine has implications for the treatment of intestinal disorders that arise due to altered ISC behavior.

Indexed as

AgingAnimalsCell Adhesion Molecules, NeuronalCell ProliferationDrosophilaDrosophila ProteinsErbB ReceptorsFemaleGene Expression Regulation, DevelopmentalIntestinesSignal TransductionStem CellsCell Adhesion Molecules, NeuronalDrosophila ProteinsErbB ReceptorsNrg protein, DrosophilaagingDrosophilaEGFRintestineL1CAMNeuroglian

Identifiers

PMID33961791
PMCPMC8190597
OpenAlexW3157836072

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.