Evidence map›Paper›PMID 33961231›Full record

ArticleGenes & genomics2021

Genomic appraisal of Klebsiella PGPB isolated from soil to enhance the growth of barley.

Sheetal Sharma, Shraddha Gang, Jorg Schumacher, Martin Buck, Meenu Saraf

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Article in Genes & genomics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
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1 · What the graph read from it

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3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Application potential ofOpen life sciences · 2026
    Article
  2. Article
  3. Article
  4. Mechanism of Salt Tolerance and Plant Growth Promotion inInternational journal of molecular sciences · 2023
    Article
4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Sheetal SharmaDepartment of Microbiology and Biotechnology, School of Sciences, Gujarat University, Ahmedabad, 380009, India.
Shraddha GangDepartment of Life Science, Faculty of Natural Sciences, Imperial College, London, SW7 2AZ, UK.
Jorg SchumacherDepartment of Life Science, Faculty of Natural Sciences, Imperial College, London, SW7 2AZ, UK. j.schumacher@imperial.ac.uk.
Martin BuckDepartment of Life Science, Faculty of Natural Sciences, Imperial College, London, SW7 2AZ, UK.
Meenu SarafDepartment of Microbiology and Biotechnology, School of Sciences, Gujarat University, Ahmedabad, 380009, India. sarafmeenu@gmail.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundPGPR has substituted chemical fertilizers to enhance the nutrient profile of the soil. Although gene encoding for PGP activity is present in PGPB their activity changes in response to conditions.

objectiveTo study comparative genomics for three Klebsiella strains and their PGPR activity in response to in vitro and soil condition.

methodsWe evaluated the activity of three Klebsiella spp. in two different conditions, specific nitrogen-deficient MS media and greenhouse experiment. Applying comparative genomics, genes encoding for PGP traits were identified from the whole-genome sequencing of the three strains. With the help of the RAST tool kit and functional annotation, a total number of genes encoding for cell wall capsule, nitrogen metabolism, sulfur genes and many other functional groups were identified. With the help of blast circular genome, similarity between GC content, pseudogene and tRNA was represented. The percentage of gene similarity of SSN1 was generated against BLAST with M5a1 and SGM81. Other methods like synteny alignment and orthologous gene clusters were applied to understand the homologous present in three strains.

resultsSSN1 was actively producing the maximum amount of ammonia 10.97 ± 0.29 µmol/mL compared to the other two strains. K. oxytoca M5a1 was considered negative for all PGP traits except ammonia production. The activity of SSN1 was showing a consistent pattern both the conditions whereas M5a1 was only active in vitro condition. Gene encoding for allantoin metabolism allD, allC, allB, allA, allE, allR, allH were identified in SSN1 and M5a1 but was absent in SGM81. The highest COG was shared between SGM81 and SSN1 predicting a maximum number of similar genes. The nif gene cluster was 98 % identical to the M5a1 strain.

conclusionsComparatively, SSN1 expressed the additional gene for various PGP traits which suggest higher efficiency of strain in nitrogen deficiency stress.

Indexed as

GenomicsEscherichia coliEscherichia coli ProteinsGenome, PlantHordeumKlebsiellaNitrogenNitrogen FixationPhosphatidate PhosphatasePhylogenyRNA, TransferSoilEscherichia coli ProteinsNitrogenPgpB protein, E coliPhosphatidate PhosphataseRNA, TransferSoilBiological nitrogen fixationComparative genomicsKlebsiellaPGPR genesPot studyRoot elongation

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.