Evidence map›Paper›PMID 33961222›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Metagenomic Assembly: Reconstructing Genomes from Metagenomes.

Zhang Wang, Jie-Liang Liang, Li-Nan Huang, Alessio Mengoni, Wen-Sheng Shu

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. Screening Host Genomic Data for Wolbachia Infections.Methods in molecular biology (Clifton, N.J.) · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Zhang WangInstitute of Ecological Science, School of Life Science, South China Normal University, Guangzhou, Guangdong Province, China.
Jie-Liang LiangInstitute of Ecological Science, School of Life Science, South China Normal University, Guangzhou, Guangdong Province, China.
Li-Nan HuangSchool of Life Sciences, Sun Yat-Sen University, Guangzhou, Guangdong Province, China. eseshln@mail.sysu.edu.cn.
Alessio MengoniDepartment of Biology, University of Florence, Florence, Italy.
Wen-Sheng ShuInstitute of Ecological Science, School of Life Science, South China Normal University, Guangzhou, Guangdong Province, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Assembly of metagenomic sequence data into microbial genomes is of critical importance for disentangling community complexity and unraveling the functional capacity of microorganisms. The rapid development of sequencing technology and novel assembly algorithms have made it possible to reliably reconstruct hundreds to thousands of microbial genomes from raw sequencing reads through metagenomic assembly. In this chapter, we introduce a routinely used metagenomic assembly workflow including read quality filtering, assembly, contig/scaffold binning, and postassembly check for genome completeness and contamination. We also describe a case study to reconstruct near-complete microbial genomes from metagenomes using our workflow.

Indexed as

MetagenomeMetagenomicsSequence Analysis, DNADatabases, GeneticHigh-Throughput Nucleotide SequencingPhylogenyResearch DesignSoftwareWorkflowAssemblyContig/scaffold binningGenome curationMetagenomics

Identifiers

PMID33961222

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.