ArticleAnalytical and bioanalytical chemistry2021
A green and facile approach to a graphene-based peroxidase-like nanozyme and its application in sensitive colorimetric detection of L-cysteine.
Article in Analytical and bioanalytical chemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Graphene-based nanozymes for revolutionizing biomedical research.RSC advances · 2025Review
- Advances of Nanozyme-Driven Multimodal Sensing Strategies in Point-of-Care Testing.Biosensors · 2025Review
- Peroxidase-like Active Cu-MOFs Nanozymes for Colorimetric Detection of Total Antioxidant Capacity in Fruits and Vegetables.Foods (Basel, Switzerland) · 2025Article
- Poly-β-cyclodextrin strengthen PrAnalytical and bioanalytical chemistry · 2024Article
- Article
- Platinum Palladium Bimetallic Nanozymes Stabilized with Vancomycin for the Sensitive Colorimetric Determination of L-cysteine.Biomolecules · 2023Article
- Ag nanozyme strengthened by folic acid: Superior peroxidase-mimicking activity and application for visual monitoring of dopamine.Analytical and bioanalytical chemistry · 2022Article
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Abstract
A facile and green approach to the preparation of peroxidase-like nanozymes by reducing and functionalizing graphene oxide (rGO) with Ganoderma polysaccharide (GP) has been achieved in this work. Our results showed that the as-fabricated nanozyme, namely rGO-GP, possessed the excellent property of simulating peroxidase with higher catalytic activity compared with GO or rGO obtained by using chitosan, which may be due to the better dispersion of rGO-GP in the solution. Steady-state kinetics studies further showed that the catalytic process conformed to Michaelis-Menten equation and ping-pong mechanism. Benefiting from the excellent peroxidase property of rGO-GP, we have also successfully established a highly sensitive and selective colorimetric detection approach to trace detection of L-cysteine (L-Cys). The limit of detection (LOD) of L-cysteine is 0.1 μM and the linear detection range is 2-30 μM. Furthermore, the nanozyme was successfully applied for detecting L-cysteine in serum. This work therefore demonstrates the advantages of rGO-GP as an effective nanozyme in both its green synthesis and detecting application.
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