Evidence map›Paper›PMID 33960726›Full record

ArticleFEBS open bio2021

De novo reconstitution of chromatin using wheat germ cell-free protein synthesis.

Yaeta Endo, Nobuaki Takemori, Szilvia K Nagy, Kei-Ichi Okimune, Rohinton Kamakaka, Hitoshi Onouchi, Taichi E Takasuka

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in FEBS open bio, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Yaeta EndoProteo-Science Center, Ehime University, Matsuyama, Japan.
Nobuaki TakemoriDivision of Proteomics Research Proteo-Science Center, Ehime University, Toon, Japan.
Szilvia K NagyDepartment of Molecular Biology, Institute of Biochemistry and Molecular Biology, Semmelweis University, Budapest, Hungary.
Kei-Ichi OkimuneResearch Faculty of Agriculture, Hokkaido University, Sapporo, Japan.
Rohinton KamakakaDepartment of Molecular Cell and Developmental Biology, University of California at Santa Cruz, CA, USA.
Hitoshi OnouchiResearch Faculty of Agriculture, Hokkaido University, Sapporo, Japan.
Taichi E TakasukaResearch Faculty of Agriculture, Hokkaido University, Sapporo, Japan.ORCID 0000-0002-4413-2924

Funding

Chromatin Domain DynamicsR01GM078068 · NIGMS · UNIVERSITY OF CALIFORNIA SANTA CRUZ · PI KAMAKAKA, ROHINTON T. · 2007 to 2018
$3.7M
NIGMS NIH HHS R01 GM078068
6 · The paper itself

Abstract

DNA is packaged with histones to form chromatin that impinges on all nuclear processes, including transcription, replication and repair, in the eukaryotic nucleus. A complete understanding of these molecular processes requires analysis of chromatin context in vitro. Here, Drosophila four core histones were produced in a native and unmodified form using wheat germ cell-free protein synthesis. In the assembly reaction, four unpurified core histones and three chromatin assembly factors (dNAP-1, dAcf1 and dISWI) were incubated with template DNA. We then assessed stoichiometry with the histones, nucleosome arrays, supercoiling and the ability of the chromatin to serve as a substrate for histone-modifying enzymes. Overall, our method provides a new avenue to produce chromatin that can be useful in a wide range of chromatin research.

Indexed as

AnimalsChromatinDNADrosophila melanogasterDrosophila ProteinsChromatinDNADrosophila ProteinsATP-dependent nucleosome assemblyhistone modificationsMg2+ chromatin isolationpost-translational chromatin assemblyunmodified and soluble histone productswheat germ cell-free protein synthesis

Identifiers

PMID33960726
PMCPMC8167859

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.