ArticlePlant biotechnology journal2021
Establishment of an Agrobacterium-mediated genetic transformation and CRISPR/Cas9-mediated targeted mutagenesis in Hemp (Cannabis Sativa L.).
Article in Plant biotechnology journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
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Who cites it
73 citing papers in PubMed, 145 citations in OpenAlex.
- An Integrated Transient Expression and UPLC-PDA Approach for Functional Genomics and Metabolic Analysis of Cannabis sativa.Biotechnology journal · 2026Article
- From Model Plants toPlants (Basel, Switzerland) · 2026Review
- Research Progress on Preparation, Transformation and Application of Protoplasts Derived from Medicinal Plants.Plants (Basel, Switzerland) · 2026Review
- Back to the roots: Cannabis sativa L. root metabolism, microbiomes, and biotechnological potential.Applied microbiology and biotechnology · 2026Review
- Genetic Advances inPlants (Basel, Switzerland) · 2026Review
- Establishment of a High-Frequency Plant Regeneration Protocol for the MultipurposePlants (Basel, Switzerland) · 2026Article
- Improving Ramie Fiber: Current Progress and Future Directions in Molecular Breeding.Plants (Basel, Switzerland) · 2026Review
- Article
- Micropropagation of Cannabis sativa: genetic and epigenetic stability assessment over multiple generations.Journal of cannabis research · 2026Article
- Callus-mediated plant regeneration and CRISPR/Cas9-targeted mutagenesis inHorticulture research · 2026Article
- Advances in the use of morphogenic regulators and peptide regenerating factors for boosting plant transformation and genome editing.Frontiers in plant science · 2026Review
- Genotype-independent de novo regeneration protocol in Cannabis sativa L. through direct organogenesis from cotyledonary nodes.Plant methods · 2025Article
- Application of a GRF-GIF chimera enhances plant regeneration for genome editing in tomato.Plant biotechnology journal · 2025Article
- Biotechnological Advances in Sanguinarine and Chelerythrine Production from Plume Poppy (Plants (Basel, Switzerland) · 2025Review
- Genome-wide characterization and expression analysis of the growth-regulating factor family in Mikania micrantha.BMC plant biology · 2025Article
- Initial shoot regeneration in the selenium hyperaccumulator Neptunia amplexicaulis and in vitro test system for selenium tolerance and accumulation.Natural products and bioprospecting · 2025Article
- Exploring Aroma and Flavor Diversity inMolecules (Basel, Switzerland) · 2025Review
- Haploid Production inBiology · 2025Review
- Nutrients and Bioactive Compounds fromInternational journal of molecular sciences · 2025Review
- Current Advancement and Future Prospects in Simplified Transformation-Based Plant Genome Editing.Plants (Basel, Switzerland) · 2025Review
13 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
13 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hemp (Cannabis sativa L.) is an annual and typically dioecious crop. Due to the therapeutic potential for human diseases, phytocannabinoids as a medical therapy is getting more attention recently. Several candidate genes involved in cannabinoid biosynthesis have been elucidated using omics analysis. However, the gene function was not fully validated due to few reports of stable transformation for Cannabis tissues. In this study, we firstly report the successful generation of gene-edited plants using an Agrobacterium-mediated transformation method in C. sativa. DMG278 achieved the highest shoot induction rate, which was selected as the model strain for transformation. By overexpressing the cannabis developmental regulator chimera in the embryo hypocotyls of immature grains, the shoot regeneration efficiency was substantially increased. We used CRISPR/Cas9 technology to edit the phytoene desaturase gene and finally generated four edited cannabis seedlings with albino phenotype. Moreover, we propagated the transgenic plants and validated the stable integration of T-DNA in cannabis genome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.