Evidence map›Paper›PMID 33959616›Full record

ArticleFrontiers in cell and developmental biology2021

An Optimized and Versatile Counter-Flow Centrifugal Elutriation Workflow to Obtain Synchronized Eukaryotic Cells.

Yongqiang Liu, Bei Nan, Junhua Niu, Geoffrey M Kapler, Shan Gao

Abstract read
In one paragraph

Article in Frontiers in cell and developmental biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Specific tracking of N-terminal clipping on histone H3 inMarine life science & technology · 2026
    Article
  2. Article
  3. Dynamic DNAGenome research · 2024
    Article
  4. Transcriptome analysis of the binucleate ciliateMolecular biology of the cell · 2023
    Article
  5. Review
  6. Anterior-posterior pattern formation in ciliates.The Journal of eukaryotic microbiology · 2022
    Review
  7. Review
  8. Cell Cycle Progression and Synchronization: An Overview.Methods in molecular biology (Clifton, N.J.) · 2022
    Review
  9. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Yongqiang LiuInstitute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, China.
Bei NanInstitute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, China.
Junhua NiuInstitute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, China.
Geoffrey M KaplerDepartment of Molecular and Cellular Medicine, Texas A&M University, College Station, TX, United States.
Shan GaoInstitute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cell synchronization is a powerful tool to understand cell cycle events and its regulatory mechanisms. Counter-flow centrifugal elutriation (CCE) is a more generally desirable method to synchronize cells because it does not significantly alter cell behavior and/or cell cycle progression, however, adjusting specific parameters in a cell type/equipment-dependent manner can be challenging. In this paper, we used the unicellular eukaryotic model organism,

Indexed as

cell cyclecounter-flow centrifugal elutriationeukaryotic cellssynchronizationTetrahymena thermophila

Identifiers

PMID33959616
PMCPMC8093812

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.