ArticleFrontiers in neuroanatomy2021
Application of the Mirror Technique for Three-Dimensional Electron Microscopy of Neurochemically Identified GABA-ergic Dendrites.
Article in Frontiers in neuroanatomy, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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3 citing papers in PubMed, 3 citations in OpenAlex.
- Dendritic synaptome of calcium-binding protein containing GABAergic interneurons in the mouse primary visual cortex.Frontiers in neural circuits · 2025Article
- Ultraplex microscopy: versatile highly-multiplexed molecular labeling and imaging across scale and resolution.bioRxiv : the preprint server for biology · 2024Article
- Application of the mirror technique for block-face scanning electron microscopy.Brain structure & function · 2022Article
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5 authors at 1 institution in 1 country.
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Abstract
In the nervous system synaptic input arrives chiefly on dendrites and their type and distribution have been assumed pivotal in signal integration. We have developed an immunohistochemistry (IH)-correlated electron microscopy (EM) method - the "mirror" technique - by which synaptic input to entire dendrites of neurochemically identified interneurons (INs) can be mapped due preserving high-fidelity tissue ultrastructure. Hence, this approach allows quantitative assessment of morphometric parameters of synaptic inputs along the whole length of dendrites originating from the parent soma. The method exploits the fact that adjoining sections have truncated or cut cell bodies which appear on the common surfaces in a mirror fashion. In one of the sections the histochemical marker of the GABAergic subtype, calbindin was revealed in cell bodies whereas in the other section the remaining part of the very same cell bodies were subjected to serial section EM to trace and reconstruct the synaptology of entire dendrites. Here, we provide exemplary data on the synaptic coverage of two dendrites belonging to the same calbindin-D
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