ArticleScientific reports2021
An individual alginate lyase is effective in the disruption of Laminaria digitata recalcitrant cell wall.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 25 citations in OpenAlex.
- Optimization of pulsed electric field pretreatment followed by cellulase hydrolysis for enhanced protein recovery and antioxidant activity from brown seaweed (Undaria pinnatifida).Bioresources and bioprocessing · 2026Article
- Ammonia oxidation and recalcitrant carbon degradation fuel mixotrophic growth in the symbiont community of a deep-sea sponge.Microbiome · 2026Article
- Alginate oligosaccharides derived from tropical brown seaweeds as sustainable alternatives to antibiotic growth promoters in poultry nutrition: Functional mechanisms and production perspectives.Veterinary world · 2026Review
- Characterization of alginate-degrading bacteria isolated from seaweed-producing areas of South Korean territory and verification of the bacteria as plant growth-promoting biofertilizer.Microbiology spectrum · 2025Article
- Recent Advances in Alginate Lyase Engineering for Efficient Conversion of Alginate to Value-Added Products.Microbial biotechnology · 2025Review
- Improving protein hydrolysis and digestibility inHeliyon · 2025Article
- Effect of Laminaria digitata dietary inclusion and CAZyme supplementation on blood cells, serum metabolites and hepatic lipids and minerals of weaned piglets.Scientific reports · 2023Article
- Mango Peel Nanofiltration Concentrates to Enhance Anaerobic Digestion of Slurry from Piglets Fed with Laminaria.Membranes · 2023Article
- Combined effects of dietary Laminaria digitata with alginate lyase on plasma metabolites and hepatic lipid, pigment and mineral composition of broilers.BMC veterinary research · 2022Article
- Effect of DietaryAnimals : an open access journal from MDPI · 2022Article
- Influence of Feeding Weaned Piglets withFoods (Basel, Switzerland) · 2022Article
- Comparing the infection biology and gene expression differences ofFrontiers in microbiology · 2022Article
- Quality Traits and Nutritional Value of Pork and Poultry Meat from Animals Fed with Seaweeds.Foods (Basel, Switzerland) · 2021Review
- The Function of CBM32 in Alginate Lyase VxAly7B on the Activity on Both Soluble Sodium Alginate and Alginate Gel.Frontiers in microbiology · 2021Article
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9 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
In the present study, 199 pre-selected Carbohydrate-Active enZymes (CAZymes) and sulfatases were assessed, either alone or in combination, to evaluate their capacity to disrupt Laminaria digitata cell wall, with the consequent release of interesting nutritional compounds. A previously characterized individual alginate lyase, belonging to the family 7 of polysaccharide lyases (PL7) and produced by Saccharophagus degradans, was shown to be the most efficient in the in vitro degradation of L. digitata cell wall. The alginate lyase treatment, compared to the control, released up to 7.11 g/L of reducing sugars (p < 0.001) and 8.59 mmol/100 g dried alga of monosaccharides (p < 0.001), and reduced cell wall fluorescence intensity by 39.1% after staining with Calcofluor White (p = 0.001). The hydrolysis of gel-forming polymer alginate by the alginate lyase treatment could prevent the trapping of fatty acids and release beneficial monounsaturated fatty acids, particularly 18:1c9 (p < 0.001), to the extracellular medium. However, no liberation of proteins (p > 0.170) or pigments (p > 0.070) was observed. Overall, these results show the ability of an individual alginate lyase, from PL7 family, to partially degrade L. digitata cell wall under physiological conditions. Therefore, this CAZyme can potentially improve the bioavailability of L. digitata bioactive compounds for monogastric diets, with further application in feed industry.
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