ArticleMolecular pharmacology2021
Automated Intracellular Pharmacological Electrophysiology for Ligand-Gated Ionotropic Receptor and Pharmacology Screening.
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Article in Molecular pharmacology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10citing papers in PubMed
0.7field-weighted citation impact, top 31% of its field
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Who cites it
10 citing papers in PubMed, 12 citations in OpenAlex.
- A modular method for high-throughput measurement of ion channel currents in cardiac myocytes.Nature protocols · 2026Review
- High-throughput multiplex voltage-clamp/current-clamp evaluation of acutely isolated neurons.Nature protocols · 2026Review
- Structural basis for channel gating and blockade in tri-heteromeric GluN1-2B-2D NMDA receptor.Neuron · 2025Article
- The evolution of patch-clamp electrophysiology: Robotic, multiplex, and dynamic.Molecular pharmacology · 2025Review
- Patch-walking, a coordinated multi-pipette patch clamp for efficiently finding synaptic connections.eLife · 2024Article
- Patch-walking: Coordinated multi-pipette patch clamp for efficiently finding synaptic connections.bioRxiv : the preprint server for biology · 2024Article
- Harnessing the potential of human induced pluripotent stem cells, functional assays and machine learning for neurodevelopmental disorders.Frontiers in neuroscience · 2024Review
- De novo CLPTM1 variants with reduced GABAEpilepsia · 2023Article
- Commonly Used Therapeutics Associated with Changes in Arousal Inhibit GABABiomolecules · 2023Article
- High-throughput combined voltage-clamp/current-clamp analysis of freshly isolated neurons.Cell reports methods · 2023Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Riley E PerszykGeorge W Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, Georgia (R.E.P., M.C.Y., C.R.F.); Department of Pharmacology and Chemical Biology, Emory University School of Medicine, Atlanta, Georgia (R.E.P., A.J., S.F.T.); Department of Molecular Genetics & Microbiology, Center for Neurogenetics, Genetics Institute, University of Florida, Gainesville, Florida (O.L.M., E.T.W.); and Department of Anesthesiology, Emory University, Atlanta, Georgia (A.J.).
Mighten C YipGeorge W Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, Georgia (R.E.P., M.C.Y., C.R.F.); Department of Pharmacology and Chemical Biology, Emory University School of Medicine, Atlanta, Georgia (R.E.P., A.J., S.F.T.); Department of Molecular Genetics & Microbiology, Center for Neurogenetics, Genetics Institute, University of Florida, Gainesville, Florida (O.L.M., E.T.W.); and Department of Anesthesiology, Emory University, Atlanta, Georgia (A.J.).
Ona L McConnellGeorge W Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, Georgia (R.E.P., M.C.Y., C.R.F.); Department of Pharmacology and Chemical Biology, Emory University School of Medicine, Atlanta, Georgia (R.E.P., A.J., S.F.T.); Department of Molecular Genetics & Microbiology, Center for Neurogenetics, Genetics Institute, University of Florida, Gainesville, Florida (O.L.M., E.T.W.); and Department of Anesthesiology, Emory University, Atlanta, Georgia (A.J.).
Eric T WangGeorge W Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, Georgia (R.E.P., M.C.Y., C.R.F.); Department of Pharmacology and Chemical Biology, Emory University School of Medicine, Atlanta, Georgia (R.E.P., A.J., S.F.T.); Department of Molecular Genetics & Microbiology, Center for Neurogenetics, Genetics Institute, University of Florida, Gainesville, Florida (O.L.M., E.T.W.); and Department of Anesthesiology, Emory University, Atlanta, Georgia (A.J.).
Andrew JenkinsGeorge W Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, Georgia (R.E.P., M.C.Y., C.R.F.); Department of Pharmacology and Chemical Biology, Emory University School of Medicine, Atlanta, Georgia (R.E.P., A.J., S.F.T.); Department of Molecular Genetics & Microbiology, Center for Neurogenetics, Genetics Institute, University of Florida, Gainesville, Florida (O.L.M., E.T.W.); and Department of Anesthesiology, Emory University, Atlanta, Georgia (A.J.) cforest@gatech.edu ajenki2@emory.edu strayne@emory.edu.
Stephen F TraynelisGeorge W Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, Georgia (R.E.P., M.C.Y., C.R.F.); Department of Pharmacology and Chemical Biology, Emory University School of Medicine, Atlanta, Georgia (R.E.P., A.J., S.F.T.); Department of Molecular Genetics & Microbiology, Center for Neurogenetics, Genetics Institute, University of Florida, Gainesville, Florida (O.L.M., E.T.W.); and Department of Anesthesiology, Emory University, Atlanta, Georgia (A.J.) cforest@gatech.edu ajenki2@emory.edu strayne@emory.edu.
Craig R ForestGeorge W Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, Georgia (R.E.P., M.C.Y., C.R.F.); Department of Pharmacology and Chemical Biology, Emory University School of Medicine, Atlanta, Georgia (R.E.P., A.J., S.F.T.); Department of Molecular Genetics & Microbiology, Center for Neurogenetics, Genetics Institute, University of Florida, Gainesville, Florida (O.L.M., E.T.W.); and Department of Anesthesiology, Emory University, Atlanta, Georgia (A.J.) cforest@gatech.edu ajenki2@emory.edu strayne@emory.edu.
University of Florida · USEmory University · USGeorgia Institute of Technology · US
Funding
Novel Platforms for Systematic Optical Control of Complex Neural Circuits In VivoR01DA029639 · NIDA · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BOYDEN, EDWARD S. · 2010 to 2023
$6.7MGlutamate receptors and human neurological diseaseR35NS111619 · NINDS · EMORY UNIVERSITY · PI Stephen F Traynelis · 2019 to 2026
$6.6MHigh-throughput robotic analysis of integrated neuronal phenotypesR01EY023173 · NEI · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BOYDEN, EDWARD S., FOREST, CRAIG · 2012 to 2016
$4.3MPsychosis-related Physiological and Neuronal Phenotypes in 22q11 Deletion SyndromeR01MH117315 · NIMH · EMORY UNIVERSITY · PI CUBELLS, JOSEPH F., DUNCAN, ERICA J · 2019 to 2023
$3.4MCharacterization of an endogenous GABA-ergic mechanism underlying hypersomniaR01NS089719 · NINDS · EMORY UNIVERSITY · PI RYE, DAVID B · 2015 to 2018
$2.4MAntibiotic-mediated improvements in vigilance: mechanisms of action of clarithromycin in hypersomnia syndromesR01NS111280 · NINDS · EMORY UNIVERSITY · PI TROTTI, LYNN MARIE · 2019 to 2023
$2.2MScalable Cell- and Circuit-Targeted ElectrophysiologyR01NS102727 · NINDS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BOYDEN, EDWARD S., FOREST, CRAIG · 2017 to 2020
$2.2MIn-vivo circuit activity measurement at single cell, sub-threshold resolutionU01MH106027 · NIMH · GEORGIA INSTITUTE OF TECHNOLOGY · PI FOREST, CRAIG, STANLEY, GARRETT B. · 2014 to 2016
$1.5MFrom Cells to Systems and Applications: Computational Neuroscience Training at EmT90DA032466 · NIDA · EMORY UNIVERSITY · PI JAEGER, DIETER, STANLEY, GARRETT B. · 2011 to 2015
$817kNEI NIH HHS R01 EY023173NIDA NIH HHS R01 DA029639NIDA NIH HHS T90 DA032466NIMH NIH HHS R01 MH117315NIMH NIH HHS U01 MH106027NINDS NIH HHS R01 NS089719NINDS NIH HHS R01 NS102727NINDS NIH HHS R01 NS111280NINDS NIH HHS R35 NS111619
Abstract
Communication between neuronal cells, which is central to brain function, is performed by several classes of ligand-gated ionotropic receptors. The gold-standard technique for measuring rapid receptor response to agonist is manual patch-clamp electrophysiology, capable of the highest temporal resolution of any current electrophysiology technique. We report an automated high-precision patch-clamp system that substantially improves the throughput of these time-consuming pharmacological experiments. The patcherBot
Indexed as
AnimalsCHO CellsCricetulusDrug Evaluation, PreclinicalHEK293 CellsHumansMiceNeuronsPatch-Clamp TechniquesPrimary Cell CultureRatsReceptors, GABA-ARoboticsReceptors, GABA-A
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.