ArticleParasites & vectors2021
Real-time PCR biochip for on-site detection of Coxiella burnetii in ticks.
Article in Parasites & vectors, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 8 citations in OpenAlex.
- Tick-borne bacterial pathogens in wildlife and their parasitizing ticks in Korea: A molecular epidemiological study.Parasites, hosts and diseases · 2026Article
- Epidemiological investigation and genetic characterization of Coxiella burnetii carried by parasitic ticks in the southeastern coastal region of China.BMC infectious diseases · 2026Article
- [Surveillance of Tick Populations in the Republic of Korea in 2024].Jugan geon-gang gwa jilbyeong · 2025Article
- Molecular Epidemiology ofAnimals : an open access journal from MDPI · 2025Article
- First Molecular Typing of Tick-DerivedTransboundary and emerging diseases · 2025Article
- Molecular surveillance of zoonotic pathogens from wild rodents in the Republic of Korea.PLoS neglected tropical diseases · 2024Article
- Distribution and Prevalence ofInfectious disease reports · 2023Review
- Genotyping ofGenes · 2022Article
- Toxoplasma gondii and Rickettsia spp. in ticks collected from migratory birds in the Republic of Korea.Scientific reports · 2022Article
- Pattern Recognition Receptors in Innate Immunity to Obligate Intracellular Bacteria.Zoonoses (Shannon, Ireland) · 2021Article
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
Abstract
backgroundQ fever, a zoonosis caused by Coxiella burnetii, has adverse effects on public health. Ticks are vectors of C. burnetii and they contribute to the transmission of the pathogen. A tool for rapid, sensitive, and accurate detection of C. burnetii from ticks is important for the prevention of Q fever.
methodsUltra-rapid real-time PCR (UR-qPCR) as a chip-based real-time PCR system was developed for the detection of C. burnetii from ticks. The UR-qPCR system was established and evaluated for the rapidity, sensitivity, and specificity of C. burnetii detection.
resultsC. burnetii was detected using UR-qPCR from 5644 larval, nymphal, and adult ticks from 408 pools collected from livestock and epidemiologically linked environments in two provinces, Gangwon and Jeju, in Korea. Ticks from three species were identified; Haemaphysalis longicornis accounted for the highest number, present in 333 of 408 pools (81.62%), followed by Haemaphysalis flava in 62 pools (15.19%) and Ixodes nipponensis in 13 pools (3.19%). The rapidity and sensitivity of PCR detection was demonstrated with the sufficient amplification and detection of approximately 56 copies of C. burnetii DNA with only 20 min of PCR amplification. The kappa value for the diagnostic agreement between UR-qPCR and stationary qPCR was in perfect agreement (κ = 1). PCR detection and sequencing indicated that C. burnetii was present in 5 of the 408 pools (1.23%), in which four pools contained H. longicornis and one pool contained H. flava. The infection rates of C. burnetii in the tick pools collected from Gangwon and Jeju Provinces were 1.70% and 0.58%, respectively. Phylogenetic analysis indicated a close relationship between the detected C. burnetii and those originating from goats, humans, and ticks in different countries, such as the USA, France, Germany, and Serbia.
conclusionsThe methods described in this study could be important for the prevention and control of Q fever in the two provinces. The UR-qPCR, with its features of mobility, sensitivity, and rapidity, is helpful for constructing early alert systems in the field for C. burnetii in ticks and could help alleviate the transmission of and economic damage due to Q fever.
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