ArticleGenome biology2021
Transgenerational effect of mutants in the RNA-directed DNA methylation pathway on the triploid block in Arabidopsis.
Article in Genome biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 23 citations in OpenAlex.
- Presence and function of small RNAs during plant reproduction.RNA biology · 2026Review
- Molecular mechanisms and biological functions of active DNA demethylation in plants.Epigenetics & chromatin · 2025Review
- Epigenetic gene regulation in plants and its potential applications in crop improvement.Nature reviews. Molecular cell biology · 2025Review
- Parental dialectic: Epigenetic conversations in endosperm.Current opinion in plant biology · 2024Review
- Epigenetic and transcriptional consequences in the endosperm of chemically induced transposon mobilization in Arabidopsis.Nucleic acids research · 2024Article
- The Biosynthesis Process of Small RNA and Its Pivotal Roles in Plant Development.International journal of molecular sciences · 2024Review
- Targeted suppression of siRNA biogenesis in Arabidopsis pollen promotes triploid seed viability.Nature communications · 2024Article
- Molecular basis and evolutionary drivers of endosperm-based hybridization barriers.Plant physiology · 2024Review
- New insights into the regulation of plant metabolism by O-acetylserine: sulfate and beyond.Journal of experimental botany · 2023Article
- Bypassing reproductive barriers in hybrid seeds using chemically induced epimutagenesis.The Plant cell · 2022Article
- Biogenesis, Trafficking, and Function of Small RNAs in Plants.Frontiers in plant science · 2022Article
- The Underlying Nature of Epigenetic Variation: Origin, Establishment, and Regulatory Function of Plant Epialleles.International journal of molecular sciences · 2021Review
- Transgenerational effect of mutants in the RNA-directed DNA methylation pathway on the triploid block in Arabidopsis.Genome biology · 2021Article
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Authors and funding
6 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHybridization of plants that differ in number of chromosome sets (ploidy) frequently causes endosperm failure and seed arrest, a phenomenon referred to as triploid block. In Arabidopsis, loss of function of NRPD1, encoding the largest subunit of the plant-specific RNA polymerase IV (Pol IV), can suppress the triploid block. Pol IV generates short RNAs required to guide de novo methylation in the RNA-directed DNA methylation (RdDM) pathway. Recent work suggests that suppression of the triploid block by mutants in RdDM components differs, depending on whether the diploid pollen is derived from tetraploid plants or from the omission in second division 1 (osd1) mutant. This study aims to understand this difference.
resultsIn this study, we find that the ability of mutants in the RdDM pathway to suppress the triploid block depends on their degree of inbreeding. While first homozygous generation mutants in RdDM components NRPD1, RDR2, NRPE1, and DRM2 have weak or no ability to rescue the triploid block, they are able to suppress the triploid block with successive generations of inbreeding. Inbreeding of nrpd1 was connected with a transgenerational loss of non-CG DNA methylation on sites jointly regulated by CHROMOMETHYLASES 2 and 3.
conclusionsOur data reveal that loss of RdDM function differs in its effect in early and late generations, which has important implications when interpreting the effect of RdDM mutants.
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