Evidence map›Paper›PMID 33956511›Full record

ArticleMolecular biology of the cell2021

Permitted and restricted steps of human kinetochore assembly in mitotic cell extracts.

Ekaterina V Tarasovetc, Praveen Kumar Allu, Robert T Wimbish, Jennifer G DeLuca, Iain M Cheeseman, Ben E Black, Ekaterina L Grishchuk

Open access · bronzeAbstract read
In one paragraph

Article in Molecular biology of the cell, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.5field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 5 citations in OpenAlex.

  1. Structure and function of the outer kinetochore.Nature reviews. Molecular cell biology · 2026
    Review
  2. Binding site maturation modulated by molecular density underlies Ndc80 binding to kinetochore receptor CENP-T.Proceedings of the National Academy of Sciences of the United States of America · 2024
    Article
  3. Article
  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Ekaterina V TarasovetcDepartment of Physiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104.
Praveen Kumar AlluDepartment of Biochemistry and Biophysics, Penn Center for Genome Integrity, Epigenetics Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104.
Robert T WimbishDepartment of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523.
Jennifer G DeLucaDepartment of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523.
Iain M CheesemanWhitehead Institute for Biomedical Research, Cambridge, MA 02142.
Ben E BlackDepartment of Biochemistry and Biophysics, Penn Center for Genome Integrity, Epigenetics Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104.
Ekaterina L GrishchukDepartment of Physiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104.
University of Pennsylvania · USColorado State University · USWhitehead Institute for Biomedical Research · US

Funding

Molecular Analysis of Kinetochore FunctionR35GM126930 · NIGMS · WHITEHEAD INSTITUTE FOR BIOMEDICAL RES · PI Iain McPherson Cheeseman · 2018 to 2026
$7.0M
Centromere identity, strength, and regulationR35GM130302 · NIGMS · UNIVERSITY OF PENNSYLVANIA · PI Ben E. Black · 2019 to 2026
$4.3M
Mechanisms of Kinetochore-Microtubule Attachment and RegulationR35GM130365 · NIGMS · COLORADO STATE UNIVERSITY · PI Jennifer G DeLuca · 2019 to 2026
$3.8M
Coupling kinetochore microtubule dynamics to chromosome motionR01GM098389 · NIGMS · UNIVERSITY OF PENNSYLVANIA · PI GRISHCHUK, EKATERINA L · 2012 to 2020
$3.1M
NIGMS NIH HHS R01 GM098389NIGMS NIH HHS R35 GM126930NIGMS NIH HHS R35 GM130302NIGMS NIH HHS R35 GM130365
6 · The paper itself

Abstract

Mitotic kinetochores assemble via the hierarchical recruitment of numerous cytosolic components to the centromere region of each chromosome. However, how these orderly and localized interactions are achieved without spurious macromolecular assemblies forming from soluble kinetochore components in the cell cytosol remains poorly understood. We developed assembly assays to monitor the recruitment of green fluorescent protein-tagged recombinant proteins and native proteins from human cell extracts to inner kinetochore components immobilized on microbeads. In contrast to prior work in yeast and

Indexed as

Cell ExtractsCentromereCentromere Protein AChromosomal Proteins, Non-HistoneChromosome SegregationCytoskeletal ProteinsGreen Fluorescent ProteinsHumansKinetochoresMicrotubule-Associated ProteinsMicrotubulesMitosisNuclear ProteinsCell ExtractsCentromere Protein Acentromere protein CChromosomal Proteins, Non-HistoneCytoskeletal ProteinsGreen Fluorescent ProteinsMicrotubule-Associated ProteinsNDC80 protein, humanNuclear Proteins

Identifiers

PMID33956511
PMCPMC8351545
OpenAlexW3157076569

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.