ArticleThe Plant cell2021
Live imaging of microtubule organization, cell expansion, and intercellular space formation in Arabidopsis leaf spongy mesophyll cells.
Article in The Plant cell, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 27 citations in OpenAlex.
- Brassinosteroid controls leaf air space patterning non-cell autonomously by promoting epidermal growth.Development (Cambridge, England) · 2026Article
- Developmental changes in epidermal anatomy, drought tolerance and biomechanics in the leaves of a tropical fern.Annals of botany · 2025Article
- Technological advances in visualizing and rewiring microtubules during plant development.Journal of experimental botany · 2025Review
- Rho GTPase overexpression impacts leaf internal architecture and mesophyll conductance in Arabidopsis.The New phytologist · 2025Article
- Phosphate promotes Arabidopsis root skewing and circumnutation through reorganisation of the microtubule cytoskeleton.The New phytologist · 2024Article
- Greater mesophyll conductance and leaf photosynthesis in the field through modified cell wall porosity and thickness via AtCGR3 expression in tobacco.Plant biotechnology journal · 2024Article
- Overwintering performance of bamboo leaves, and establishment of mathematical model for the distribution and introduction prediction of bamboos.Frontiers in plant science · 2023Article
- 3-D reconstruction of rice leaf tissue for proper estimation of surface area of mesophyll cells and chloroplasts facing intercellular airspaces from 2-D section images.Annals of botany · 2022Article
- Double Puzzle: Morphogenesis of the Bi-Layered Leaf Adaxial Epidermis ofPlants (Basel, Switzerland) · 2022Article
- Localized growth and remodelling drives spongy mesophyll morphogenesis.Journal of the Royal Society, Interface · 2022Article
- CLASP balances two competing cell division plane cues during leaf development.Nature plants · 2022Article
- Structural organization of the spongy mesophyll.The New phytologist · 2022Article
- Live-imaging provides an atlas of cellular growth dynamics in the stamen.Plant physiology · 2022Article
- Laticifer growth pattern is guided by cytoskeleton organization.Frontiers in plant science · 2022Article
- Riddled with holes: Understanding air space formation in plant leaves.PLoS biology · 2021Review
- A Sight on Single-Cell Transcriptomics in Plants Through the Prism of Cell-Based Computational Modeling Approaches: Benefits and Challenges for Data Analysis.Frontiers in genetics · 2021Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Leaf spongy mesophyll cells form an interconnected network of branched cells and intercellular spaces to maximize the surface area available for light capture and photosynthetic gas exchange. To investigate the morphogenetic events leading to cell separation and branching in Arabidopsis thaliana, we used mesophyll-specific promoters to facilitate imaging of mesophyll cell shape and microtubule (MT) organization over multiple spatiotemporal scales without interference from the overlying epidermal cells. We show that cells enlarge by selective expansion of cell wall regions in contact with intercellular spaces. Cell-cell contacts remain relatively fixed in size, forming the termini of interconnecting branches. Surprisingly, classic schizogeny (de-adhesion of neighboring cells) is relatively infrequent, being related to the local topology of cell junctions during early expansion. Intercellular spaces cue the position of stable MT bundles, which in turn promote efficient dilation of intercellular spaces and cell branching. Our data provide insights into mesophyll morphogenesis and MT organization and lay the groundwork for future investigations.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.