ArticleThe Plant cell2021
Distinct identities of leaf phloem cells revealed by single cell transcriptomics.
Article in The Plant cell, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 169 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
169 citing papers in PubMed, 341 citations in OpenAlex.
- A single-nucleus atlas of ligular region uncovers brassinosteroid-driven cell fate transitions controlling leaf angle in maize.Nature communications · 2026Article
- Decoding the landscape of cell-type-specific co-expressed transcription factors in soybean.Nature plants · 2026Article
- Systemic acquired resistance: an emerging role for jasmonates in local signal biogenesis, translocation and distal signal decoding.The New phytologist · 2026Review
- Genome-Wide Identification of the Soybean UMAMIT Family and Functional Analysis ofInternational journal of molecular sciences · 2026Article
- Positional grammar of transcription factor binding partitions developmental and stress-response regulation in plants.Nature communications · 2026Article
- GhNAC47 activates GhSKS6-mediated cell wall remodeling to promote cotton chemical defoliation.The New phytologist · 2026Article
- The Contribution of Phloem Metabolism to Leaf Respiration: New Insights Into an Old Problem.Plant, cell & environment · 2026Article
- Single-nucleus RNA sequencing reveals a spatiotemporal pattern of HScience China. Life sciences · 2026Article
- The architecture of salt tolerance: A multi-scale view of sodium transport in plants.Plant communications · 2026Review
- Single-nucleus sequencing and spatial metabolomics analysis reveal the regulatory mechanism of ginkgolic acid biosynthesis in the episperm ofHorticulture research · 2026Article
- Single-cell RNA-sequencing profiles reveal the developmental landscape of hawthorn leaves.BMC plant biology · 2026Article
- Heads Up: Transcriptomics Reveal Functional Roles of Cannabis Glandular Trichome Stalks.Plants (Basel, Switzerland) · 2026Article
- Deciphering Cell-Type-Specific Transcriptional Regulation in Tomato Leaves Through Ensemble Machine Learning and Single-Cell Transcriptomics.Plants (Basel, Switzerland) · 2026Article
- Advances in Functional Genomics and Biotechnology for Enhancing Therapeutic Potential of Medicinal Plants.International journal of molecular sciences · 2026Review
- Transcription Profiling of Potato Leaves in Response to Heat Stress at Single-Cell Resolution.Plant biotechnology journal · 2026Article
- Single-Cell Transcriptomics Reveals FLS2-Dependent Hypoxia Signaling and ERF13-Mediated Transcription During flg22-Triggered Immunity.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Cell-type-specific gating of gene regulatory modules as a hallmark of early immune responses in Arabidopsis leaves.The New phytologist · 2026Article
- Laminar patterning transcription factors orchestrate spatial metabolite partitioning in Capsicum fruit.Nature plants · 2026Article
- Single-cell and spatial transcriptomics in Phragmites australis reveal the association of B chromosomes with plant invasiveness.Genome biology · 2026Article
- Stress drives plasticity in leaf ageing transcriptional dynamics in Arabidopsis thaliana.Nature plants · 2026Article
109 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The leaf vasculature plays a key role in solute translocation. Veins consist of at least seven distinct cell types, with specific roles in transport, metabolism, and signaling. Little is known about leaf vascular cells, in particular the phloem parenchyma (PP). PP effluxes sucrose into the apoplasm as a basis for phloem loading, yet PP has been characterized only microscopically. Here, we enriched vascular cells from Arabidopsis leaves to generate a single-cell transcriptome atlas of leaf vasculature. We identified at least 19 cell clusters, encompassing epidermis, guard cells, hydathodes, mesophyll, and all vascular cell types, and used metabolic pathway analysis to define their roles. Clusters comprising PP cells were enriched for transporters, including SWEET11 and SWEET12 sucrose and UmamiT amino acid efflux carriers. We provide evidence that PP development occurs independently from ALTERED PHLOEM DEVELOPMENT, a transcription factor required for phloem differentiation. PP cells have a unique pattern of amino acid metabolism activity distinct from companion cells (CCs), explaining differential distribution/metabolism of amino acids in veins. The kinship relation of the vascular clusters is strikingly similar to the vein morphology, except for a clear separation of CC from the other vascular cells including PP. In summary, our single-cell RNA-sequencing analysis provides a wide range of information into the leaf vasculature and the role and relationship of the leaf cell types.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.