Evidence map›Paper›PMID 33955335›Full record

ArticlePlant signaling & behavior2021

Virus-induced gene silencing (VIGS) analysis shows involvement of the LsSTPK gene in lettuce (

Lu Wang, Yang Wu, Wei Du, Ziqi Yan, Zhengyang Qi, Wenkun Tang, Yingyan Han, Chaojie Liu, Shuangxi Fan, Jinghong Hao

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant signaling & behavior, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.7field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
  3. Frontiers in plant science · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 2 countries.

Lu WangBeijing Key Laboratory for Agricultural Application and New Technology, National Demonstration Center for Experimental Plant Production Education, College of Plant Science and Technology, Beijing University of Agriculture, Beijing, China.
Yang WuBeijing Key Laboratory for Agricultural Application and New Technology, National Demonstration Center for Experimental Plant Production Education, College of Plant Science and Technology, Beijing University of Agriculture, Beijing, China.
Wei DuBeijing Key Laboratory for Agricultural Application and New Technology, National Demonstration Center for Experimental Plant Production Education, College of Plant Science and Technology, Beijing University of Agriculture, Beijing, China.
Ziqi YanUniversity of Arizona, Tucson, Arizona, USA.
Zhengyang QiBeijing Key Laboratory for Agricultural Application and New Technology, National Demonstration Center for Experimental Plant Production Education, College of Plant Science and Technology, Beijing University of Agriculture, Beijing, China.
Wenkun TangBeijing Key Laboratory for Agricultural Application and New Technology, National Demonstration Center for Experimental Plant Production Education, College of Plant Science and Technology, Beijing University of Agriculture, Beijing, China.
Yingyan HanBeijing Key Laboratory for Agricultural Application and New Technology, National Demonstration Center for Experimental Plant Production Education, College of Plant Science and Technology, Beijing University of Agriculture, Beijing, China.
Chaojie LiuBeijing Key Laboratory for Agricultural Application and New Technology, National Demonstration Center for Experimental Plant Production Education, College of Plant Science and Technology, Beijing University of Agriculture, Beijing, China.
Shuangxi FanBeijing Key Laboratory for Agricultural Application and New Technology, National Demonstration Center for Experimental Plant Production Education, College of Plant Science and Technology, Beijing University of Agriculture, Beijing, China.
Jinghong HaoBeijing Key Laboratory for Agricultural Application and New Technology, National Demonstration Center for Experimental Plant Production Education, College of Plant Science and Technology, Beijing University of Agriculture, Beijing, China.
Beijing University of Agriculture · CNUniversity of Arizona · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

To determine the effect of the serine/threonine protein kinase (STPK) gene on leaf lettuce bolting, we utilized virus-induced gene silencing (VIGS) using the TRV vector to silence the target gene. The 'GB30' leaf lettuce cultivar was the test material, and the methods included gene cloning, bioinformatics analysis, quantitative real-time PCR (qRT-PCR) and VIGS. LsSTPK, was cloned from the 'GB30' leaf lettuce cultivar via reverse transcription-polymerase chain reaction (RT-PCR). qRT-PCR analysis showed that the expression of LsSTPK in the stem of leaf lettuce was significantly greater than that in the roots and leaves, and after high-temperature treatment, the gene expression in the stems in the experimental group was markedly lower than that in the control groups. Following LsSTPK silencing via the VIGS method, the stem length in the treatment group was significantly greater than that in the blank and negative control groups, and the contents of auxin (IAA), GA3 and abscisic acid (ABA) in the treatment group were greater than those in the other two groups. Flower bud differentiation occurred in the treatment group but not in the control group. The above findings suggested that LsSTPK inhibits the bolting of leaf lettuce under high-temperature conditions.

Indexed as

Gene SilencingCloning, MolecularGenes, PlantGenetic VectorsHot TemperatureLactucaPlant VirusesProtein Serine-Threonine KinasesReal-Time Polymerase Chain ReactionProtein Serine-Threonine KinasesBoltingfunctional analysisleaf lettucelsstpkvigs

Identifiers

PMID33955335
PMCPMC8205037
OpenAlexW3158616969

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.