Evidence map›Paper›PMID 33954954›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Imaging of Polar and Nonpolar Lipids Using Desorption Electrospray Ionization/Post-photoionization Mass Spectrometry.

Chengyuan Liu, Fei Qi, Yang Pan

Abstract readComparative Study
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
2.3field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Chengyuan LiuNational Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei, China.
Fei QiKey Laboratory for Power Machinery and Engineering of Ministry of Education, Shanghai Jiao Tong University, Shanghai, China.
Yang PanNational Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei, China. panyang@ustc.edu.cn.
University of Science and Technology of China · CNShanghai Jiao Tong University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Desorption electrospray ionization mass spectrometry imaging (DESI-MSI) can record 2D distribution of polar lipids in tissue slices at ambient condition. However, sensitivity of DESI-MSI for nonpolar lipids is restricted by low ionization efficiency and severe ion suppression. Here, a compact post-photoionization assembly combined with DESI (DESI/PI) was developed for simultaneous imaging polar and nonpolar lipids in tissue sections by switching off/on a portable krypton lamp. Compared with DESI, higher signal intensities of nonpolar compounds could be detected with DESI/PI. We describe the fabrication, optimization, implementation, and data transformation for imaging both the polar and nonpolar lipids in mouse brain tissue using an Agilent 6224 Accurate-Mass TOF mass spectrometer. More than ten nonpolar lipids including cholesterol and GalCer lipids were detected by DESI/PI in the positive ion mode, compared with that by DESI. In the negative-ion mode, ion yields of DESI/PI for lipids (HexCer, PE, and PE-P) were also increased by several folds.

Indexed as

Brain ChemistryAnimalsLipidsMiceMolecular ImagingPhotochemical ProcessesSpectrometry, Mass, Electrospray IonizationLipidsDesorption electrospray ionizationMass spectrometry imagingPost-photoionization

Identifiers

PMID33954954
OpenAlexW3158743857

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.