Evidence map›Paper›PMID 33954949›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Shotgun Lipidomic Analysis of Leishmania Cells.

Kai Zhang, Fong-Fu Hsu

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
7.9field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 2 institutions in 1 country.

Kai ZhangDepartment of Biological Sciences, Texas Tech University, Lubbock, TX, USA.
Fong-Fu HsuMass Spectrometry Resource, Division of Endocrinology, Metabolism, and Lipid Research, Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA. fhsu@wustl.edu.
Texas Tech University · USWashington University in St. Louis · US

Funding

Washington University Nutrition Obesity Research CenterP30DK056341 · NIDDK · WASHINGTON UNIVERSITY · PI Dominic N Reeds · 1999 to 2026
$30.2M
WU P&FP30DK020579 · NIDDK · WASHINGTON UNIVERSITY · PI Clay F. Semenkovich · 2013 to 2026
$27.1M
A Biomedical Mass Spectrometry Resource: Ongoing Driving Biomedical ProjectsR24GM136766 · NIGMS · WASHINGTON UNIVERSITY · PI GROSS, MICHAEL L · 2020 to 2022
$2.3M
Exploring an Essential and Dangerous Pathway in Leishmania ParasitesR01AI099380 · NIAID · TEXAS TECH UNIVERSITY · PI ZHANG, KAI · 2013 to 2017
$1.8M
Roles of Plasmenylethanolamine Biosynthesis in Leishmania virulenceR15AI076909 · NIAID · TEXAS TECH UNIVERSITY · PI ZHANG, KAI · 2008 to 2008
$206k
NIAID NIH HHS R01 AI099380NIAID NIH HHS R15 AI076909NIDDK NIH HHS P30 DK020579NIDDK NIH HHS P30 DK056341NIGMS NIH HHS R24 GM136766
6 · The paper itself

Abstract

To understand the interplay of lipids between Leishmania promastigotes, amastigotes, and vertebrate host cells, a robust method for cultivating Leishmania parasites, lipid extraction, and shotgun lipidomic analysis with loop injection is described. This book chapter provides the step-by-step workflow to guide readers from sample preparation to the global lipid analysis by multiple stage mass spectrometry with high resolution and tandem quadrupole mass spectrometric approaches toward studying the metabolomic roles that lipids may play in Leishmania parasite infections.

Indexed as

LeishmaniaLipidomicsLipidsMolecular StructureTandem Mass SpectrometryWorkflowLipidsGlycerophospholipidsHigh-resolution mass spectrometryLeishmania lipidsSphingolipids

Identifiers

PMID33954949
PMCPMC13431451
OpenAlexW3158291581

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.