Evidence map›Paper›PMID 33954847›Full record

ArticleClinical rheumatology2021

Plasma exosomes derived from patients with intestinal Behçet's syndrome induce intestinal epithelial cell pyroptosis.

Cheng-Cheng Hou, Hai-Fen Ma, Jing-Fen Ye, Dan Luo, Hua-Fang Bao, Jian-Long Guan

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In one paragraph

Article in Clinical rheumatology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
0.5field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 9 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Cheng-Cheng HouDepartment of Rheumatology and Immunology, Huadong Hospital Affiliated to Fudan University, Shanghai, China.
Hai-Fen MaDepartment of Rheumatology and Immunology, Huadong Hospital Affiliated to Fudan University, Shanghai, China.
Jing-Fen YeDepartment of Rheumatology and Immunology, Huadong Hospital Affiliated to Fudan University, Shanghai, China.
Dan LuoDepartment of Rheumatology and Immunology, Huadong Hospital Affiliated to Fudan University, Shanghai, China.
Hua-Fang BaoDepartment of Rheumatology and Immunology, Huadong Hospital Affiliated to Fudan University, Shanghai, China.
Jian-Long GuanDepartment of Rheumatology and Immunology, Huadong Hospital Affiliated to Fudan University, Shanghai, China. jianlong_guan@126.com.
Huadong Hospital · CNFudan University · CN

Funding

the Clinical Science Innovation Program of Shanghai Shenkang Hospital Development Center SHDC12017129the General Program of National Natural Science Foundation of China 81871276
6 · The paper itself

Abstract

objectivesIntestinal Behçet's syndrome (IBS) has high morbidity and mortality rates with serious complications. The purpose of this study was to investigate the expression of pyroptosis-related proteins in the intestinal tissues of IBS patients and explore the role of plasma exosomes derived from IBS patients in the pyroptosis of intestinal epithelial cells.

methodImmunohistochemistry was used to investigate the expression of nucleotide-binding domain-like receptor protein 3 (NLRP3), caspase-1, and gasdermin D (GSDMD). Quantitative real-time PCR was employed to measure the mRNA levels of IL-1β and IL-18 in the intestinal tissues. Plasma exosomes were isolated and observed by transmission electron microscopy. The exosomes were co-cultured with intestinal epithelial cells in vitro. Western blot was used to measure the expression of pyroptosis-related proteins including NLRP3, full-length GSDMD, N-terminal GSDMD, pro-caspase-1, and cleaved caspase-1. The levels of IL-1β and IL-18 were detected by enzyme-linked immunosorbent assay. Cell death was measured by using the lactate dehydrogenase (LDH) release assay.

resultsExpression of NLRP3 (12.2% ± 1.2%, 8.1% ± 0.9%, t = 4.692, p = 0.009), caspase-1 (24.6% ± 2.1%, 4.2% ± 1.8%, t = 12.842, p = 0.000), and GSDMD (16.6% ± 1.9%, 9.8% ± 1.3%, t = 5.194, p = 0.007) were significantly increased in the intestinal tissues of patients with IBS compared with normal control (NC) group, respectively. The relative mRNA levels of IL-1β (t = 4.308, p = 0.005) and IL-18 (t = 3.096, p = 0.021) in the intestinal tissues were significantly higher in IBS patients than in NC group, while the protein levels of IL-1β (t = 3.873, p = 0.018) and IL-18 (t = 4.389, p = 0.012) were also significantly increased, which was consistent with the results of the relative mRNA levels. Moreover, we found that exosomes from IBS patients significantly induced pyroptosis of intestinal epithelial cells via the activation of NLRP3 inflammasome in vitro experiments.

conclusionsPlasma exosomes derived from IBS patients may induce pyroptosis of intestinal epithelial cells via the activation of NLRP3 inflammasome. Key Points •The role of exosomes in IBS is first reported in this study. • In this study, we explored the mechanism that plasma exosomes derived from IBS patients may induce pyroptosis of intestinal epithelial cells via the activation of NLRP3 inflammasome.

Indexed as

Behcet SyndromeExosomesEpithelial CellsHumansInflammasomesNLR Family, Pyrin Domain-Containing 3 ProteinPyroptosisInflammasomesNLR Family, Pyrin Domain-Containing 3 ProteinBehçet’s diseaseExosomesNLRP3 inflammasomePyroptosis

Identifiers

PMID33954847
OpenAlexW3158135587

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.