Evidence map›Paper›PMID 33953532›Full record

ArticleMolecular vision2021

Differences in activation of intracellular signaling in primary human retinal endothelial cells between isoforms of VEGFA 165.

Wendelin Dailey, Roberto Shunemann, Fang Yang, Megan Moore, Austen Knapp, Peter Chen, Mrinalini Deshpande, Brandon Metcalf, Quentin Tompkins, Alvaro E Guzman and 2 more

Open access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in Molecular vision, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.6field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

Wendelin DaileyEye Research Institute, Oakland University, Rochester, MI.
Roberto ShunemannEye Research Institute, Oakland University, Rochester, MI.
Fang YangEye Research Institute, Oakland University, Rochester, MI.
Megan MooreEye Research Institute, Oakland University, Rochester, MI.
Austen KnappEye Research Institute, Oakland University, Rochester, MI.
Peter ChenEye Research Institute, Oakland University, Rochester, MI.
Mrinalini DeshpandeEye Research Institute, Oakland University, Rochester, MI.
Brandon MetcalfEye Research Institute, Oakland University, Rochester, MI.
Quentin TompkinsEye Research Institute, Oakland University, Rochester, MI.
Alvaro E GuzmanEye Research Institute, Oakland University, Rochester, MI.
Jennifer FeliskyEye Research Institute, Oakland University, Rochester, MI.
Kenneth P MittonEye Research Institute, Oakland University, Rochester, MI.
Smith-Kettlewell Eye Research Institute · USOakland University · US

Funding

VEGF'S LONGITUDINAL EFFECT ON THE RETINA AND RETINAL VASCULATURE IN VIVOR15EY025089 · NEI · OAKLAND UNIVERSITY · PI MITTON, KENNETH PATRICK · 2016 to 2016
$449k
NEI NIH HHS R15 EY025089
6 · The paper itself

Abstract

Purpose: There are reports that a b-isoform of vascular endothelial growth factor-A 165 (VEGFA Methods: To determine full dose-response curves for the activation of MAPK (ERK1/2), AKT, and VEGFR2, direct in-cell western assays were developed using primary HRMECs. Potential differences in dose-response effects on gene expression markers related to endothelial cell and leukocyte adhesion ( Results: Activation dose-response analysis revealed much stronger activation of MAPK, AKT, and VEGFR2 by the a-isoform at lower doses. MAPK activation in primary HRMECs displayed a sigmoidal dose-response to a range of VEGFA Conclusions: In primary HRMECs, VEGFA

Indexed as

Claudin-5Endothelium, VascularE-SelectinGene Expression RegulationHumansImmunoblottingIntercellular Adhesion Molecule-1Mitogen-Activated Protein Kinase KinasesOccludinPolymerase Chain ReactionProtein IsoformsProto-Oncogene Proteins c-aktRetinal VesselsSignal TransductionTranscriptional ActivationVascular Cell Adhesion Molecule-1Claudin-5CLDN5 protein, humanE-SelectinICAM1 protein, humanIntercellular Adhesion Molecule-1KDR protein, humanMitogen-Activated Protein Kinase KinasesOccludinOCLN protein, humanProtein IsoformsProto-Oncogene Proteins c-aktSELE protein, humanVascular Cell Adhesion Molecule-1Vascular Endothelial Growth Factor AVascular Endothelial Growth Factor Receptor-2VEGFA protein, human

Identifiers

PMID33953532
PMCPMC8092446
OpenAlexW3159305299

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.