ArticleCommunications biology2021
Overlapping roles of spliceosomal components SF3B1 and PHF5A in rice splicing regulation.
Article in Communications biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 23 citations in OpenAlex.
- Determination of trunk neural crest cell fate and susceptibility to splicing perturbation by the DLC1-SF3B1-PHF5A splicing complex.Nature communications · 2025Article
- A simple method to visualize pre-mRNA splicing with the naked eye using a genetically encoded visual splicing reporter.Plant physiology · 2024Article
- Stress-induced nuclear translocation of ONAC023 improves drought and heat tolerance through multiple processes in rice.Nature communications · 2024Article
- SCR106 splicing factor modulates abiotic stress responses by maintaining RNA splicing in rice.Journal of experimental botany · 2024Article
- Synthetic directed evolution for targeted engineering of plant traits.Frontiers in plant science · 2024Review
- CRISPR-Cas systems and applications for crop bioengineering.Frontiers in bioengineering and biotechnology · 2024Review
- Co-expression network of heat-response transcripts: A glimpse into how splicing factors impact rice basal thermotolerance.Frontiers in molecular biosciences · 2023Article
- Synthetic evolution of herbicide resistance using a T7 RNAP-based random DNA base editor.Life science alliance · 2022Article
- The Rice Serine/Arginine Splicing Factor RS33 Regulates Pre-mRNA Splicing during Abiotic Stress Responses.Cells · 2022Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The SF3B complex, a multiprotein component of the U2 snRNP of the spliceosome, plays a crucial role in recognizing branch point sequence and facilitates spliceosome assembly and activation. Several chemicals that bind SF3B1 and PHF5A subunits of the SF3B complex inhibit splicing. We recently generated a splicing inhibitor-resistant SF3B1 mutant named SF3B1 GEX1A RESISTANT 4 (SGR4) using CRISPR-mediated directed evolution, whereas splicing inhibitor-resistant mutant of PHF5A (Overexpression-PHF5A GEX1A Resistance, OGR) was generated by expressing an engineered version PHF5A-Y36C. Global analysis of splicing in wild type and these two mutants revealed the role of SF3B1 and PHF5A in splicing regulation. This analysis uncovered a set of genes whose intron retention is regulated by both proteins. Further analysis of these retained introns revealed that they are shorter, have a higher GC content, and contain shorter and weaker polypyrimidine tracts. Furthermore, splicing inhibition increased seedlings sensitivity to salt stress, consistent with emerging roles of splicing regulation in stress responses. In summary, we uncovered the functions of two members of the plant branch point recognition complex. The novel strategies described here should be broadly applicable in elucidating functions of splicing regulators, especially in studying the functions of redundant paralogs in plants.
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