ArticleMicrobes and environments2021
RNA Viral Metagenome Analysis of Subnanogram dsRNA Using Fragmented and Primer Ligated dsRNA Sequencing (FLDS).
Article in Microbes and environments, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 26 citations in OpenAlex.
- Molecular characterization of a novel botourmiavirus infecting Leptosphaeria biglobosa.Archives of virology · 2026Article
- Genome characterization of two novel mitoviruses and a negative-sense single-stranded RNA mycovirus from the phytopathogenic fungus Clarireedia jacksonii.Archives of virology · 2026Article
- Identification of hot spring Obelisk-like RNA replicons and expanded diversity of the Obelisk superfamily.Nature communications · 2026Article
- Identification of a novel toti-like virus symbiotic with Giardia duodenalis.Archives of virology · 2026Article
- Identification of Obelisk-like covalently closed circular RNA replicon in hot springs by double-stranded RNA sequencing and expansion of the diversity of the Obelisk superfamily.bioRxiv : the preprint server for biology · 2025Article
- Molecular characterization of a polymycovirus in Leptosphaeria biglobosa.Archives of virology · 2025Article
- "Paraxenoviridae", a putative family of ubiquitous marine bacteriophages with double-stranded RNA genomes.bioRxiv : the preprint server for biology · 2025Article
- "Paraxenoviridae", a putative family of globally distributed marine bacteriophages with double-stranded RNA genomes.The ISME journal · 2025Article
- Mycoviruses from Aspergillus fungi involved in fermentation of dried bonito.Virus research · 2024Article
- Reexamining the Mycovirome ofViruses · 2024Article
- New lineages of RNA viruses from clinical isolates ofmSphere · 2024Article
- Molecular and biological characterization of a novel partitivirus from Talaromyces pinophilus.Virus research · 2024Article
- Double-stranded RNA sequencing reveals distinct riboviruses associated with thermoacidophilic bacteria from hot springs in Japan.Nature microbiology · 2024Article
- Seven new mycoviruses identified from isolated ascomycetous macrofungi.Virus research · 2024Article
- Molecular and biological characterization of a partitivirus fromThe Journal of general virology · 2023Article
- Experimental verification of strain-dependent relationship between mycovirus and its fungal host.iScience · 2023Article
- Distinct groups of RNA viruses associated with thermoacidophilic bacteria.bioRxiv : the preprint server for biology · 2023Article
- Current trends in RNA virus detection through metatranscriptome sequencing data.FEBS open bio · 2023Review
- Identification of novel RNA mycoviruses from wild mushroom isolates in Japan.Virus research · 2023Article
- The First Identification of a Narnavirus in Bigyra, a Marine Protist.Microbes and environments · 2023Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fragmented and primer ligated dsRNA sequencing (FLDS) is a sequencing method applicable to long double-stranded RNA (dsRNA) that enables the complete genome sequencing of both double- and single-stranded RNA viruses. However, the application of this method on a low amount of dsRNA has been hindered by adaptor dimer formation during cDNA amplification and sequence library preparation. We herein developed FLDS ver. 3 by optimizing the terminal modification of an oligonucleotide adaptor and the conditions of adaptor ligation. We also examined the concentration of Mg
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