ArticleCurrent biology : CB2021
Direct visualization of epithelial microvilli biogenesis.
Article in Current biology : CB, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed, 58 citations in OpenAlex.
- Myosin-9b Controls Epithelial Brush Border Architecture through Motility-Dependent RhoA Signaling.bioRxiv : the preprint server for biology · 2026Article
- Structural plasticity of mouse intestinal microvilli revealed by tissue-level cryo-ET.Biophysics reports · 2026Article
- ARHGAP18 complexes with both YAP and Merlin and is required for basal actin bundles.Molecular biology of the cell · 2026Article
- Steady-state epithelial apical flatness is characterized by MLCK morphodynamics and asynchronous CaMolecular biology of the cell · 2026Article
- Integrated Multi-Omic Analysis Identifies Altered Colonic Brush Border Profile as a Key Feature of Microscopic Colitis.United European gastroenterology journal · 2026Article
- Brush border intermicrovillar adhesion limits bacteria attachment to the small intestine brush border.bioRxiv : the preprint server for biology · 2025Article
- Osteocyte Dendrites: How Do They Grow, Mature, and Degenerate in Mineralized Bone?Cytoskeleton (Hoboken, N.J.) · 2025Review
- Apical clathrin-coated pits control the location, timing, and scale of microvillar growth.Cell reports · 2025Article
- The Rho effector ARHGAP18 coordinates a Hippo pathway feedback loop through YAP and Merlin to regulate the cytoskeleton and epithelial cell polarity.bioRxiv : the preprint server for biology · 2025Article
- Protrusion-Derived Extracellular Vesicles (PD-EVs) and Their Diverse Origins: Key Players in Cellular Communication, Cancer Progression, and T Cell Modulation.Biology of the cell · 2025Review
- Insulin receptor tyrosine kinase substrate in health and disease (Review).Molecular medicine reports · 2025Review
- Prominosomes - a particular class of extracellular vesicles containing prominin-1/CD133?Journal of nanobiotechnology · 2025Review
- Non-Invasive Nanometer Resolution Assessment of Cell-Soft Hydrogel System Mechanical Properties by Scanning Ion Conductance Microscopy.International journal of molecular sciences · 2024Article
- Organization of a cytoskeletal superstructure in the apical domain of intestinal tuft cells.The Journal of cell biology · 2024Article
- Heterochromatin formation and remodeling by IRTKS condensates counteract cellular senescence.The EMBO journal · 2024Article
- Host-derived CEACAM-laden vesicles engage enterotoxigenicProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Multi-Omics Profiles of Small Intestine Organoids in Reaction to Breast Milk and Different Infant Formula Preparations.Nutrients · 2024Article
- Host-derived CEACAM-laden vesicles engage enterotoxigenicbioRxiv : the preprint server for biology · 2024Article
- Photoreceptor calyceal processes accompany the developing outer segment, adopting a stable length despite a dynamic core.Journal of cell science · 2024Article
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Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Microvilli are actin-bundle-supported surface protrusions that play essential roles in diverse epithelial functions. To develop our understanding of microvilli biogenesis, we used live imaging to directly visualize protrusion growth at early stages of epithelial differentiation. Time-lapse data revealed that specific factors, including epidermal growth factor pathway substrate 8 (EPS8) and insulin-receptor tyrosine kinase substrate (IRTKS) (also known as BAIAP2L1), appear in diffraction-limited puncta at the cell surface and mark future sites of microvillus growth. New core actin bundles elongate from these puncta in parallel with the arrival of ezrin and subsequent plasma membrane encapsulation. In addition to de novo growth, we also observed that new microvilli emerge from pre-existing protrusions. Moreover, we found that nascent microvilli can also collapse, characterized first by loss of membrane wrapping and ezrin enrichment, followed by a sharp decrease in distal tip EPS8 and IRTKS levels, and ultimately disassembly of the core actin bundle itself. These studies are the first to offer a temporally resolved microvillus growth mechanism and highlight factors that participate in this process; they also provide important insights on the growth of apical specializations that will likely apply to diverse epithelial contexts.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.