ArticleJournal of the American Society for Mass Spectrometry2021
A Semiautomated Paramagnetic Bead-Based Platform for Isobaric Tag Sample Preparation.
Article in Journal of the American Society for Mass Spectrometry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 37 citations in OpenAlex.
- High-Throughput Proteomics Sample Preparation Using a 96-Channel Pipettor and Magnetic Pin Device.Journal of proteome research · 2026Article
- BRD4-mediated ER membrane contact creates functionally distinct mitochondrial subtypes.Molecular cell · 2026Article
- PBK/TOPK mediates Ikaros, Aiolos and CTCF displacement from mitotic chromosomes and alters chromatin accessibility at selected C2H2-zinc finger protein binding sites.Nature communications · 2025Article
- Development of an Adaptive, Economical, and Easy-to-Use SP3-TMT Automated Sample Preparation Workflow for Quantitative Proteomics.Journal of proteome research · 2025Article
- Expanding the landscape of aging via orbitrap astral mass spectrometry and tandem mass tag integration.Nature communications · 2025Article
- Investigating the effects of liquid handling robot pipetting speed on yeast growth and gene expression using growth assays and RNA-seq.microPublication biology · 2025Article
- Achieving a 35-Plex Tandem Mass Tag Reagent Set through Deuterium Incorporation.Journal of proteome research · 2024Article
- Proteome-wide abundance profiling of yeast deletion strains for GET pathway members using sample multiplexing.Proteomics · 2024Article
- A proximity proteomics pipeline with improved reproducibility and throughput.Molecular systems biology · 2024Article
- A proximity proteomics pipeline with improved reproducibility and throughput.bioRxiv : the preprint server for biology · 2024Article
- Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input.Cell chemical biology · 2024Article
- Automated Sample Preparation Workflow for Tandem Mass Tag-Based Proteomics.Journal of the American Society for Mass Spectrometry · 2023Article
- A proteome-wide atlas of drug mechanism of action.Nature biotechnology · 2023Article
- Enriching Cysteine-Containing Peptides Using a Sulfhydryl-Reactive Alkylating Reagent with a Phosphonic Acid Group and Immobilized Metal Affinity Chromatography.Journal of proteome research · 2023Article
- Isobaric labeling: Expanding the breadth, accuracy, depth, and diversity of sample multiplexing.Proteomics · 2022Article
- FeJournal of proteomics · 2022Article
- Proximity labeling for investigating protein-protein interactions.Methods in cell biology · 2022Article
- Shotgun Proteomics Sample Processing Automated by an Open-Source Lab Robot.Journal of visualized experiments : JoVE · 2021Article
- Strategies for mass spectrometry-based phosphoproteomics using isobaric tagging.Expert review of proteomics · 2021Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The development of streamlined and high-throughput sample processing workflows is important for capitalizing on emerging advances and innovations in mass spectrometry-based applications. While the adaptation of new technologies and improved methodologies is fast paced, automation of upstream sample processing often lags. Here we have developed and implemented a semiautomated paramagnetic bead-based platform for isobaric tag sample preparation. We benchmarked the robot-assisted platform by comparing the protein abundance profiles of six common parental laboratory yeast strains in triplicate TMTpro16-plex experiments against an identical set of experiments in which the samples were manually processed. Both sets of experiments quantified similar numbers of proteins and peptides with good reproducibility. Using these data, we constructed an interactive website to explore the proteome profiles of six yeast strains. We also provide the community with open-source templates for automating routine proteomics workflows on an opentrons OT-2 liquid handler. The robot-assisted platform offers a versatile and affordable option for reproducible sample processing for a wide range of protein profiling applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.