ArticleCurrent protocols2021
Immunohistochemical and Genetic Labeling of Hairy and Glabrous Skin Innervation.
Article in Current protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
3 citing papers in PubMed, 16 citations in OpenAlex.
- Progress on three-dimensional visualizing skin architecture with multiple immunofluorescence staining and tissue-clearing approaches.Histology and histopathology · 2025Review
- Mentalis nerve branches supplying the lower lip revisited: a study of human fetuses and donated elderly cadavers.Surgical and radiologic anatomy : SRA · 2024Article
- Follicle-innervating Aδ-low threshold mechanoreceptive neurons form receptive fields through homotypic competition.Neural development · 2023Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Cutaneous innervation is an essential component of the mammalian sensory nervous system. During development, genetically and morphologically diverse subtypes of sensory neurons use distinct molecular pathways to innervate end organs or form free nerve endings in glabrous and hairy skin. Peripheral neurons can be damaged by acute injury or degenerate due to chronic conditions including diabetes and chemotherapy, leading to peripheral neuropathy. The analysis of skin and cutaneous innervation can be applied to many research endeavors, from developmental neuroscience to pharmaceutical testing. Due to the natural hydrophobicity and heterogenous makeup of the skin (dense, keratinized cells as well as sparse, extracellular-matrix-bound cells), its histological analysis presents unique challenges compared to that of many other tissues. This series of protocols describes histological methods for generalized immunohistochemistry and subtype-specific genetic labeling of sensory neurons in mouse skin in both whole-mount and section formats. We provide detailed methodology of tissue preparation for hairy and glabrous skin, several types of labeling, and counting of hair follicles in flat-mounted mouse skin. © 2021 Wiley Periodicals LLC. Basic Protocol 1: Cryosectioning and immunostaining of mouse hairy skin Alternate Protocol 1: Alternate method for preparation and fixation of mouse hairy skin Basic Protocol 2: Sectioning of mouse paw glabrous skin Basic Protocol 3: Whole-mount immunolabeling of mouse skin Basic Protocol 4: Sparse labeling of skin-innervating neurons with a Cre-dependent membrane-bound alkaline phosphatase reporter Alternate Protocol 2: Sparse labeling of skin-innervating neurons with a Cre-dependent fluorescent reporter Basic Protocol 5: Oil Red O staining of skin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.