Evidence map›Paper›PMID 33950498›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Application of Label-Free Proteomics for Quantitative Analysis of Urothelial Carcinoma and Cystitis Tissue.

Kathrin E Witzke, Frederik Großerueschkamp, Klaus Gerwert, Barbara Sitek

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Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Kathrin E WitzkeMedizinisches Proteom-Center (MPC) Medical Faculty, Ruhr-University Bochum, Bochum, Germany.
Frederik GroßerueschkampBiospectroscopy, Center for Protein Diagnostics (PRODI), Ruhr-Universität Bochum, Bochum, Germany.
Klaus GerwertBiospectroscopy, Center for Protein Diagnostics (PRODI), Ruhr-Universität Bochum, Bochum, Germany.
Barbara SitekMedizinisches Proteom-Center (MPC) Medical Faculty, Ruhr-University Bochum, Bochum, Germany. barbara.sitek@rub.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A label-free approach based on a highly reproducible and stable workflow allows for quantitative proteome analysis . Due to advantages compared to labeling methods, the label-free approach has the potential to measure unlimited samples from clinical specimen monitoring and comparing thousands of proteins. The presented label-free workflow includes a new sample preparation technique depending on automatic annotation and tissue isolation via FTIR-guided laser microdissection, in-solution digestion, LC-MS/MS analyses, data evaluation by means of Proteome Discoverer and Progenesis software, and verification of differential proteins. We successfully applied this workflow in a proteomics study analyzing human cystitis and high-grade urothelial carcinoma tissue regarding the identification of a diagnostic tissue biomarker. The differential analysis of only 1 mm

Indexed as

ProteomeProteomicsSpectrometry, Mass, Electrospray IonizationTandem Mass SpectrometryChromatography, High Pressure LiquidCystitisHumansLaser Capture MicrodissectionNeoplasm ProteinsResearch DesignSpectroscopy, Fourier Transform InfraredUrinary Bladder NeoplasmsUrotheliumNeoplasm ProteinsProteomeAHNAK2Bladder cancerFTIR imagingLabel-free proteomicsLaser microdissectionUrothelial cell carcinoma (UCC)

Identifiers

PMID33950498

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.