ArticleMolecular biology and evolution2021
De Novo Mutation Rate Variation and Its Determinants in Chlamydomonas.
Article in Molecular biology and evolution, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 30 citations in OpenAlex.
- Stabilizing and directional selection shape the genetic variation of gene expression in the transcriptome of Chlamydomonas.Molecular biology and evolution · 2026Article
- Flipping the switch on some of the slowest mutating genomes: Direct measurements of plant mitochondrial and plastid mutation rates in msh1 mutants.PLoS genetics · 2025Article
- Flipping the switch on some of the slowest mutating genomes: Direct measurements of plant mitochondrial and plastid mutation rates inbioRxiv : the preprint server for biology · 2025Article
- Engineering the green algae Chlamydomonas incerta for recombinant protein production.PloS one · 2025Article
- Short tandem repeats delineate gene bodies across eukaryotes.Nature communications · 2024Article
- Establishing the green algaebioRxiv : the preprint server for biology · 2024Article
- Variation in the Spectrum of New Mutations among Inbred Strains of Mice.Molecular biology and evolution · 2024Article
- H3K4me1 recruits DNA repair proteins in plants.The Plant cell · 2024Article
- The Effects of De Novo Mutation on Gene Expression and the Consequences for Fitness in Chlamydomonas reinhardtii.Molecular biology and evolution · 2024Article
- Time-resolved, integrated analysis of clonally evolving genomes.PLoS genetics · 2023Article
- The divergence of mutation rates and spectra across the Tree of Life.EMBO reports · 2023Review
- Experimental estimates of germline mutation rate in eukaryotes: a phylogenetic meta-analysis.Evolution letters · 2023Article
- Shifts in mutation spectra enhance access to beneficial mutations.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Rates and spectra of de novo structural mutations inGenome research · 2023Article
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Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
De novo mutations are central for evolution, since they provide the raw material for natural selection by regenerating genetic variation. However, studying de novo mutations is challenging and is generally restricted to model species, so we have a limited understanding of the evolution of the mutation rate and spectrum between closely related species. Here, we present a mutation accumulation (MA) experiment to study de novo mutation in the unicellular green alga Chlamydomonas incerta and perform comparative analyses with its closest known relative, Chlamydomonas reinhardtii. Using whole-genome sequencing data, we estimate that the median single nucleotide mutation (SNM) rate in C. incerta is μ = 7.6 × 10-10, and is highly variable between MA lines, ranging from μ = 0.35 × 10-10 to μ = 131.7 × 10-10. The SNM rate is strongly positively correlated with the mutation rate for insertions and deletions between lines (r > 0.97). We infer that the genomic factors associated with variation in the mutation rate are similar to those in C. reinhardtii, allowing for cross-prediction between species. Among these genomic factors, sequence context and complexity are more important than GC content. With the exception of a remarkably high C→T bias, the SNM spectrum differs markedly between the two Chlamydomonas species. Our results suggest that similar genomic and biological characteristics may result in a similar mutation rate in the two species, whereas the SNM spectrum has more freedom to diverge.
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