ArticlePlant cell reports2021
A simple and efficient agroinfiltration method for transient gene expression in Citrus.
Article in Plant cell reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 38 citations in OpenAlex.
- Comprehensive Optimization of Agroinfiltration in Transient Expression System in Nicotiana tabacum with RUBY Visible Reporter.Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections · 2026Article
- Rye sprout as a host for the rapid transient production of a recombinant protein using a geminivirus DNA-containing expression vector.Plant biotechnology (Tokyo, Japan) · 2025Article
- An Efficient Agrobacterium-Mediated Transient Transformation System Using In Vitro Embryo-Derived Seedlings for Gene Function Elucidation inPlants (Basel, Switzerland) · 2025Article
- A high-efficiency transient expression system mediated by Agrobacterium tumefaciens in Spinacia oleracea leaves.Plant methods · 2024Article
- Robust soybean leaf agroinfiltration.Plant cell reports · 2024Article
- Critical parameters for robustPlant direct · 2024Article
- Transient expression of anti-HrpE scFv antibody reduces the hypersensitive response in non-host plant against bacterial phytopathogen Xanthomonas citri subsp. citri.Scientific reports · 2024Article
- ABA-CsABI5-CsCalS11 module upregulates Callose deposition of citrus infected withHorticulture research · 2024Article
- Agroinfiltration for Enhanced Transgene Expression in Coffee Leaves (Coffea arabica L.).Methods in molecular biology (Clifton, N.J.) · 2024Article
- The establishment of transient expression systems and their application for gene function analysis of flavonoid biosynthesis in Carthamus tinctorius L.BMC plant biology · 2023Article
- Reasons and riddance of Agrobacterium tumefaciens overgrowth in plant transformation.Transgenic research · 2023Review
- ERF9 of Poncirus trifoliata (L.) Raf. undergoes feedback regulation by ethylene and modulates cold tolerance via regulating a glutathione S-transferase U17 gene.Plant biotechnology journal · 2022Article
- Citrus Genetic Transformation: An Overview of the Current Strategies and Insights on the New Emerging Technologies.Frontiers in plant science · 2021Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
key messageMicrowounding pre-treatment facilitates agroinfiltration and transient gene expression in hard-to-agroinfiltrate citrus varieties. Agrobacterium infiltration is a widely used method for transient expression studies in plants, but this method is not used extensively in citrus because of its low efficiency. In this study, we developed an easy, cheap, and reliable agroinfiltration method for transient gene expression in citrus. A microneedle roller was used to create microscopic wounds in the leaf epidermis to facilitate agroinfiltration. Several optimization parameters were explored in this study, including the density of wounds per cm
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