ArticleScientific reports2021
Microdroplet-based system for culturing of environmental microorganisms using FNAP-sort.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Microdroplet-based high-throughput screening for antagonistic bacteria targeting penaeid shrimp pathogenic Vibrio harveyi.Scientific reports · 2026Article
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- Draft genome sequence ofMicrobiology resource announcements · 2026Article
- Draft genome sequence ofMicrobiology resource announcements · 2026Article
- Ultrahigh-throughput screening of heterogeneous vancomycin-intermediate Staphylococcus aureus based on fluorescence-activated droplet sorting.BMC medicine · 2025Article
- A droplet microfluidic strategy for cultivation, investigation, and high-throughput isolation of mouse gut microbiome bacteria.Applied and environmental microbiology · 2025Article
- Dielectrophoresis for Isolating Low-Abundance Bacteria Obscured by Impurities in Environmental Samples.Marine biotechnology (New York, N.Y.) · 2025Article
- Microbial single-cell applications under anoxic conditions.Applied and environmental microbiology · 2024Review
- Passive Droplet Microfluidic Platform for High-Throughput Screening of Microbial Proteolytic Activity.Analytical chemistry · 2024Article
- A Droplet-Based Microfluidic Platform for High-Throughput Culturing of Yeast Cells in Various Conditions.Micromachines · 2024Article
- Clear zone formation in microdroplets for high-throughput screening for lactic acid bacteria.Frontiers in microbiology · 2024Article
- Water-in-oil droplet-mediated method for detecting and isolating infectious bacteriophage particles via fluorescent staining.Frontiers in microbiology · 2023Article
- Article
- Droplet digital microfluidic system for screening filamentous fungi based on enzymatic activity.Microsystems & nanoengineering · 2022Article
- Emerging microfluidic technologies for microbiome research.Frontiers in microbiology · 2022Review
- Live Biosensors for Ultrahigh-Throughput Screening of Antimicrobial Activity against Gram-Negative Bacteria.Antibiotics (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Droplet microfluidics has emerged as a powerful technology for improving the culturing efficiency of environmental microorganisms. However, its widespread adoption has been limited due to considerable technical challenges, especially related to identification and manipulation of individual growth-positive droplets. Here, we combined microfluidic droplet technology with on-chip "fluorescent nucleic acid probe in droplets for bacterial sorting" (FNAP-sort) for recovery of growth-positive droplets and droplet microdispensing to establish an end-to-end workflow for isolation and culturing of environmental microbes. As a proof-of-concept, we demonstrate the ability of our technique to yield high-purity cultures of rare microorganisms from a representative complex environmental microbiome. As our system employs off-the-shelf commercially available equipment, we believe that it can be readily adopted by others and may thus find widespread use toward culturing the high proportion of as-of-yet uncultured microorganisms in different biomes.
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Registered trials
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