ArticleProceedings of the National Academy of Sciences of the United States of America2021
Optofluidic multiplex detection of single SARS-CoV-2 and influenza A antigens using a novel bright fluorescent probe assay.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 45 citations in OpenAlex.
- Ultrafast Dense Immobilization of Noble Metal Nanoparticles on Customizable Multifunctional Polymer Microspheres for Heterogeneous Catalysis and Multiplexed Biodetection.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Deep learning-enabled filter-free fluorescence microscope.Science advances · 2025Article
- Internet-enabled lab-on-a-chip technology for education.Scientific reports · 2024Article
- Bead-based microfluidic platforms for multiplex and ultrasensitive immunoassays in clinical diagnosis and treatment.Mechanobiology in medicine · 2024Review
- Label-free and amplification-free viral RNA quantification from primate biofluids using a trapping-assisted optofluidic nanopore platform.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- On-site airborne pathogen detection for infection risk mitigation.Chemical Society reviews · 2023Review
- Dual optofluidic distributed feedback dye lasers for multiplexed biosensing applications.Scientific reports · 2023Article
- Microparticle-Based Detection of Viruses.Biosensors · 2023Review
- Adaptive time modulation technique for multiplexed on-chip particle detection across scales.Optica · 2023Article
- Diagnostics and analysis of SARS-CoV-2: current status, recent advances, challenges and perspectives.Chemical science · 2023Review
- Comparison of Illumination Methods for Flow-Through Optofluidic Biosensors.Micromachines · 2023Article
- Machine learning at the edge for AI-enabled multiplexed pathogen detection.Scientific reports · 2023Article
- Article
- Rapid Detection of SARS-CoV-2 Variants of Concern by Genomic Surveillance Techniques.Advances in experimental medicine and biology · 2023Article
- Review
- Evaluation of Variant-Specific Peptides for Detection of SARS-CoV-2 Variants of Concern.Journal of proteome research · 2022Article
- All-in-One Optofluidic Chip for Molecular Biosensing Assays.Biosensors · 2022Article
- Prospects of NIR fluorescent nanosensors for green detection of SARS-CoV-2.Sensors and actuators. B, Chemical · 2022Review
- Microfluidics-Based Biosensing Platforms: Emerging Frontiers in Point-of-Care Testing SARS-CoV-2 and Seroprevalence.Biosensors · 2022Review
- FPGA Integrated Optofluidic Biosensor for Real-Time Single Biomarker Analysis.IEEE photonics journal · 2022Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The urgency for the development of a sensitive, specific, and rapid point-of-care diagnostic test has deepened during the ongoing COVID-19 pandemic. Here, we introduce an ultrasensitive chip-based antigen test with single protein biomarker sensitivity for the differentiated detection of both severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and influenza A antigens in nasopharyngeal swab samples at diagnostically relevant concentrations. The single-antigen assay is enabled by synthesizing a brightly fluorescent reporter probe, which is incorporated into a bead-based solid-phase extraction assay centered on an antibody sandwich protocol for the capture of target antigens. After optimization of the probe release for detection using ultraviolet light, the full assay is validated with both SARS-CoV-2 and influenza A antigens from clinical nasopharyngeal swab samples (PCR-negative spiked with target antigens). Spectrally multiplexed detection of both targets is implemented by multispot excitation on a multimode interference waveguide platform, and detection at 30 ng/mL with single-antigen sensitivity is reported.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.