ArticleMicrobiome2021
Synergistic action of the gut microbiota in environmental RNA interference in a leaf beetle.
Article in Microbiome, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 65 citations in OpenAlex.
- Gut bacteria prime and reinforce mature leaf oviposition preference in beetles via plant mediated feedback.Nature communications · 2026Article
- Evaluating the potential of RNA interference for control of striped cucumber beetle, Acalymma vittatum (Fabricius) (Coleoptera: Chrysomelidae).Pest management science · 2026Article
- Article
- Gut microbiota variation drives differential performance in leaf beetles across host plants.Microbiome · 2025Article
- Silencing theInsects · 2025Article
- Microbial Symbiosis in Lepidoptera: Analyzing the Gut Microbiota for Sustainable Pest Management.Biology · 2025Review
- The essential role of gut microbiota in dsRNA-mediated pest control of the phytophagous ladybird beetle, Henosepilachna vigintioctopunctata.NPJ biofilms and microbiomes · 2025Article
- Review
- Bacillus secretes nucleases to degrade dsRNA, thereby reducing host's susceptibility to RNAi.NPJ biofilms and microbiomes · 2025Article
- Blue light restores functional circadian clocks in eyeless cave spiders.Science advances · 2025Article
- Article
- Gut bacteria facilitate leaf beetles in adapting to dietary specialization by enhancing larval fitness.NPJ biofilms and microbiomes · 2024Article
- Enrichment of novel entomopathogenic Pseudomonas species enhances willow resistance to leaf beetles.Microbiome · 2024Article
- A faster killing effect of plastid-mediated RNA interference on a leaf beetle through induced dysbiosis of the gut bacteria.Plant communications · 2024Article
- Exploring the challenges of RNAi-based strategies for crop protection.Advanced biotechnology · 2024Review
- The Expression and Function ofInsects · 2024Article
- The pivotal roles of gut microbiota in insect plant interactions for sustainable pest management.NPJ biofilms and microbiomes · 2023Review
- Composition and Diversity of Gut Bacterial Community in Different Life Stages of a Leaf Beetle Gastrolina depressa.Microbial ecology · 2023Article
- Double-stranded RNA (dsRNA) technology to control forest insect pests and fungal pathogens: challenges and opportunities.Functional & integrative genomics · 2023Review
- Cloning and functional analysis of the molting gene CYP302A1 of Daphnia sinensis.Frontiers in zoology · 2023Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRNA interference (RNAi) has emerged as an efficient tool to control insect pests. When insects ingest double-stranded RNAs (dsRNAs) targeted against essential genes, strong gene silencing and mortality can be induced. To exert their function, dsRNA molecules must pass through the insect's gut and enter epithelial cells and/or the hemolymph. Gut bacteria are known to play multifarious roles in food digestion and nutrition, and confer protection against pathogens and parasites. Whether there is a cross talk between gut bacteria and ingested dsRNAs and whether the microbiome affects RNAi efficiency are unknown.
resultsHere, using a leaf beetle gut microbiota system, we investigated whether gut bacteria interact with dsRNA molecules and how the gut microbiota affects RNAi responses in insects. We first showed that the leaf beetle Plagiodera versicolora (Coleoptera) is highly susceptible to RNAi. We then demonstrated that ingestion of dsRNAs by non-axenic P. versicolora larvae results in (i) significantly accelerated mortality compared with axenic larvae, and (ii) overgrowth and dysbiosis of the gut microbiota. The latter may be caused by bacterial utilization of dsRNA degradation products. Furthermore, we found that Pseudomonas putida, a gut bacterium of P. versicolora, acts as major accelerator of the death of P. versicolora larvae by transitioning from commensal to pathogenic lifestyle.
conclusionsThe present study illuminates the complex interplay between lethal dsRNA, the insect host, and its gut microbiota. The ingestion of dsRNA by the leaf beetle caused a dysbiosis of gut bacterial community, and the dsRNA degradation products by host insect preferentially promoted the growth of an entomopathogenic bacterium, which accelerated dsRNA lethality to the insect. Our findings reveal a synergistic role of the gut microbiota in dsRNA-induced mortality of pest insects, and provide new insights in the mechanisms of RNAi-based pest control. Video abstract.
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