ArticlePharmaceutics2021
Angiogenic Properties of Concentrated Growth Factors (CGFs): The Role of Soluble Factors and Cellular Components.
Article in Pharmaceutics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.
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Who cites it
31 citing papers in PubMed, 1 synthesis or guideline pooled it, 45 citations in OpenAlex.
- Assessment of the efficacy of autologous blood preparations in maxillary sinus floor elevation surgery: a systematic review and meta-analysis.BMC oral health · 2024Pooled it
- Concentrated growth factor in oral bone regeneration: A narrative review.Regenerative therapy · 2026Review
- Autologous concentrated growth factors promote M2 macrophage polarization to accelerate wound healing after anal fistula surgery by activating the AKT/mTOR pathway.BMC gastroenterology · 2026Article
- Synergistic application of concentrated growth factor and bone perforation technique in bisphosphonate-related-osteonecrosis of the jaw.BMC oral health · 2026Article
- Research on Injectable Hydrogel GelMA-HA-CGF Loaded with Dental Pulp Stem Cells (DPSC) for Periodontal Regeneration.ACS omega · 2026Article
- Autologous Platelet Concentrates and Photobiomodulation as Biologically Active Modifiers of Hard and Soft Tissue Healing: A Randomised Controlled Trial.Journal of functional biomaterials · 2026Article
- Biological impact of adding albumin and silver nanoparticles to concentrated growth factors.BMC oral health · 2026Article
- Animal-free matrix alternative for three-dimensionalFrontiers in toxicology · 2026Article
- Evolution and mechanistic insights of platelet-derived products in temporomandibular joint regeneration.Frontiers in cell and developmental biology · 2026Review
- Recent Advances in Mechanobiology-Mediated In Situ Tissue Engineering.International journal of biomaterials · 2026Review
- Adipose-Derived Stem Cells Pretreated With Phototherapy Promote HUVECs Migration and Angiogenesis by Mediating EYA1 Activation.Physiological research · 2025Article
- The impact of natural and engineered extracellular vesicles on post-myocardial infarction angiogenesis.Stem cell research & therapy · 2025Review
- CGF-Conditioned Medium Modulates Astrocytic Differentiation and Invasiveness in U87MG Glioblastoma Cells.Biology · 2025Article
- Concentrated Growth Factor Induces ER Stress and Apoptosis by Increasing Ceramide Generation in Selected Tumour Cell Lines.Journal of cellular and molecular medicine · 2025Article
- Cells Derived from Concentrated Growth Factor Exhibit a Multilineage Differentiation Capacity.International journal of molecular sciences · 2025Article
- Innate immunity and wound repair: The platelet-rich fibrin advantage.World journal of biological chemistry · 2025Article
- A clinical study on the preservation of pulp sensitivity in affected teeth with jaw cysts using concentrated growth factor: a retrospective Cross-sectional study.Scientific reports · 2025Article
- Clinical Studies on the Application of Concentrated Growth Factors for Enhancing the Recovery From Flap Ischemia-Reperfusion Injuries.Journal of cosmetic dermatology · 2025Article
- Article
- Autologous Growth Factor-Rich Concentrate (GFC) Injection in Non-union of Fractures: A Quasi-experimental Study.Indian journal of orthopaedics · 2024Article
Corrections and comments
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Authors and funding
13 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Blood-derived concentrated growth factors (CGFs) represent a novel autologous biomaterial with promising applications in regenerative medicine. Angiogenesis is a key factor in tissue regeneration, but the role played by CGFs in vessel formation is not clear. The purpose of this study was to characterize the angiogenic properties of CGFs by evaluating the effects of its soluble factors and cellular components on the neovascularization in an in vitro model of angiogenesis. CGF clots were cultured for 14 days in cell culture medium; after that, CGF-conditioned medium (CGF-CM) was collected, and soluble factors and cellular components were separated and characterized. CGF-soluble factors, such as growth factors (VEGF and TGF-β1) and matrix metalloproteinases (MMP-2 and -9), were assessed by ELISA. Angiogenic properties of CGF-soluble factors were analyzed by stimulating human cultured endothelial cells with increasing concentrations (1%, 5%, 10%, or 20%) of CGF-CM, and their effect on cell migration and tubule-like formation was assessed by wound healing and Matrigel assay, respectively. The expression of endothelial angiogenic mediators was determined using qRT-PCR and ELISA assays. CGF-derived cells were characterized by immunostaining, qRT-PCR and Matrigel assay. We found that CGF-CM, consisting of essential pro-angiogenic factors, such as VEGF, TGF-β1, MMP-9, and MMP-2, promoted endothelial cell migration; tubule structure formation; and endothelial expression of multiple angiogenic mediators, including growth factors, chemokines, and metalloproteinases. Moreover, we discovered that CGF-derived cells exhibited features such as endothelial progenitor cells, since they expressed the CD34 stem cell marker and endothelial markers and participated in the neo-angiogenic process. In conclusion, our results suggest that CGFs are able to promote endothelial angiogenesis through their soluble and cellular components and that CGFs can be used as a biomaterial for therapeutic vasculogenesis in the field of tissue regeneration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.