Evidence map›Paper›PMID 33946302›Full record

ArticleSensors (Basel, Switzerland)2021

Sensitivity Validation of EWOD Devices for Diagnosis of Early Mortality Syndrome (EMS) in Shrimp Using Colorimetric LAMP-XO Technique.

Kreeta Sukthang, Jantana Kampeera, Chakrit Sriprachuabwong, Wansika Kiatpathomchai, Eakkachai Pengwang, Adisorn Tuantranont, Wishsanuruk Wechsatol

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Sensors (Basel, Switzerland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.7field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 7 citations in OpenAlex.

  1. Magnetic Bead Manipulation in Microfluidic Chips for Biological Application.Cyborg and bionic systems (Washington, D.C.) · 2023
    Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Kreeta SukthangDepartment of Mechanical Engineering, King Mongkut's University of Technology Thonburi, 126 Prachautid Rd., Bandmod, Thungkru, Bangkok 10140, Thailand.
Jantana KampeeraBioengineering and Sensing Technology Research Team, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand.
Chakrit SriprachuabwongGraphene and Printed Electronics for Dual-Use Applications Research Division (GPERD), National Science and Technology Development Agency (NSTDA), Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand.
Wansika KiatpathomchaiBioengineering and Sensing Technology Research Team, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand.
Eakkachai PengwangInstitute of Field Robotics, King Mongkut's University of Technology Thonburi, 126 Prachautid Rd., Bandmod, Thungkru, Bangkok 10140, Thailand.
Adisorn TuantranontGraphene and Printed Electronics for Dual-Use Applications Research Division (GPERD), National Science and Technology Development Agency (NSTDA), Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand.
Wishsanuruk WechsatolDepartment of Mechanical Engineering, King Mongkut's University of Technology Thonburi, 126 Prachautid Rd., Bandmod, Thungkru, Bangkok 10140, Thailand.
King Mongkut's University of Technology Thonburi · THNational Science and Technology Development Agency · THNational Center for Genetic Engineering and Biotechnology · TH

Funding

Thailand Research Fund RSA5680026
6 · The paper itself

Abstract

Electrowetting-on-dielectric (EWOD) is a microfluidic technology used for manipulating liquid droplets at microliter to nanoliter scale. EWOD has the ability to facilitate the accurate manipulation of liquid droplets, i.e., transporting, dispensing, splitting, and mixing. In this work, EWOD fabrication with suitable and affordable materials is proposed for creating EWOD lab-on-a-chip platforms. The EWOD platforms are applied for the diagnosis of early mortality syndrome (EMS) in shrimp by utilizing the colorimetric loop-mediated isothermal amplification method with pH-sensitive xylenol orange (LAMP-XO) diagnosis technique. The qualitative sensitivity is observed by comparing the limit of detection (LOD) while performing the LAMP-XO diagnosis test on the proposed lab-on-a-chip EWOD platform, alongside standard LAMP laboratory tests. The comparison results confirm the reliability of EMS diagnosis on the EWOD platform with qualitative sensitivity for detecting the EMS DNA plasmid concentration at 10

Indexed as

ElectrowettingMicrofluidic Analytical TechniquesColorimetryMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesReproducibility of Resultscolorimetric LAMP–XOdetection sensitivitydroplet manipulationelectrowetting-on-dielectric (EWOD)lab-on-a-chip (LOC)

Identifiers

PMID33946302
PMCPMC8124682
OpenAlexW3159812697

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.