ArticleWorld journal of microbiology & biotechnology2021
Development of a new Agrobacterium-mediated transformation system based on a dual auxotrophic approach in the filamentous fungus Aspergillus oryzae.
Article in World journal of microbiology & biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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8 citing papers in PubMed, 12 citations in OpenAlex.
- The ApCtf1β2-ApCUT3 Module Mediates Host Cuticle Penetration and Modulates Immune Responses Thereby Influencing Blight Resistance in Bambusa pervariabilis × Dendrocalamopsis grandis.Plant, cell & environment · 2026Article
- Enhanced production of ergothioneine in Aspergillus oryzae.Applied microbiology and biotechnology · 2025Article
- Expression pattern, subcellular localization ofApplied and environmental microbiology · 2025Article
- AdvancingBioengineering (Basel, Switzerland) · 2024Review
- Construction of a NewJournal of microbiology and biotechnology · 2024Article
- Establishment of RNA Interference Genetic Transformation System and Functional Analysis of FlbA Gene inInternational journal of molecular sciences · 2023Article
- A newly constructed Agrobacterium-mediated transformation system based on the hisB auxotrophic marker for genetic manipulation in Aspergillus niger.Archives of microbiology · 2023Article
- Construction of gene modification system with highly efficient and markerless forFrontiers in microbiology · 2022Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Genetic engineering of the filamentous fungus Aspergillus oryzae still requires more suitable selection markers for fungal transformation. Our previous work has shown that Agrobacterium tumefaciens-mediated transformation (ATMT) based on the uridine/uracil auxotrophic mechanism with pyrG as the selection marker is very efficient for gene transfer in A. oryzae. In the present study, we delete the hisB gene, which is essential for histidine biosynthesis, in A. oryzae via homologous recombination and demonstrate that hisB is a reliable selection marker for genetic transformation of this fungus. Under optimal conditions, the ATMT efficiency of the histidine auxotrophic A. oryzae reached 515 transformants per 10
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