Evidence map›Paper›PMID 33944774›Full record

ArticleParasite (Paris, France)2021

A novel loop-mediated isothermal amplification-lateral-flow-dipstick (LAMP-LFD) device for rapid detection of Toxoplasma gondii in the blood of stray cats and dogs.

Yangji Xue, Qingming Kong, Haojie Ding, Chengzuo Xie, Bin Zheng, Xunhui Zhuo, Jianzu Ding, Qunbo Tong, Di Lou, Shaohong Lu and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Parasite (Paris, France), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed, 1 pooled it
5.7field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 1 synthesis or guideline pooled it, 38 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 1 institution in 1 country.

Yangji XueDepartment of Immunity and Biochemistry, Institute of Parasitic Disease, Zhejiang Academy of Medical Sciences 310013 Hangzhou PR China - Hangzhou Medical College 310053 Hangzhou PR China.ORCID 0000-0003-0918-6844
Qingming KongDepartment of Immunity and Biochemistry, Institute of Parasitic Disease, Zhejiang Academy of Medical Sciences 310013 Hangzhou PR China - Hangzhou Medical College 310053 Hangzhou PR China.
Haojie DingDepartment of Immunity and Biochemistry, Institute of Parasitic Disease, Zhejiang Academy of Medical Sciences 310013 Hangzhou PR China - Hangzhou Medical College 310053 Hangzhou PR China.
Chengzuo XieDepartment of Immunity and Biochemistry, Institute of Parasitic Disease, Zhejiang Academy of Medical Sciences 310013 Hangzhou PR China - Hangzhou Medical College 310053 Hangzhou PR China.
Bin ZhengDepartment of Immunity and Biochemistry, Institute of Parasitic Disease, Zhejiang Academy of Medical Sciences 310013 Hangzhou PR China - Hangzhou Medical College 310053 Hangzhou PR China.
Xunhui ZhuoDepartment of Immunity and Biochemistry, Institute of Parasitic Disease, Zhejiang Academy of Medical Sciences 310013 Hangzhou PR China - Hangzhou Medical College 310053 Hangzhou PR China.
Jianzu DingDepartment of Immunity and Biochemistry, Institute of Parasitic Disease, Zhejiang Academy of Medical Sciences 310013 Hangzhou PR China - Hangzhou Medical College 310053 Hangzhou PR China.
Qunbo TongDepartment of Immunity and Biochemistry, Institute of Parasitic Disease, Zhejiang Academy of Medical Sciences 310013 Hangzhou PR China - Hangzhou Medical College 310053 Hangzhou PR China.
Di LouDepartment of Immunity and Biochemistry, Institute of Parasitic Disease, Zhejiang Academy of Medical Sciences 310013 Hangzhou PR China - Hangzhou Medical College 310053 Hangzhou PR China.
Shaohong LuDepartment of Immunity and Biochemistry, Institute of Parasitic Disease, Zhejiang Academy of Medical Sciences 310013 Hangzhou PR China - Hangzhou Medical College 310053 Hangzhou PR China.
Hangjun LvDepartment of Immunity and Biochemistry, Institute of Parasitic Disease, Zhejiang Academy of Medical Sciences 310013 Hangzhou PR China - Hangzhou Medical College 310053 Hangzhou PR China.
Zhejiang Academy of Medical Sciences · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Toxoplasma gondii is an obligate intracellular protozoan parasite that causes toxoplasmosis and threatens warm-blooded animal and human health worldwide. Simple and applicable diagnostic methods are urgently needed to guide development of effective approaches for prevention of toxoplasmosis. Most molecular diagnostic tools for T. gondii infection require high technical skills, sophisticated equipment, and a controlled lab environment. In this study, we developed a loop-mediated isothermal amplification-lateral-flow-dipstick (LAMP-LFD) assay that specifically targets the 529 bp for detecting T. gondii infection. This novel portable device is universal, fast, user-friendly, and guarantees experimental sensitivity as well as low risk of aerosol contamination. Our LAMP-LFD assay has a detection limit of 1 fg of T. gondii DNA, and shows no cross-reaction with other parasitic pathogens, including Cryptosporidium parvum, Leishmania donovani, and Plasmodium vivax. We validated the developed assay by detecting T. gondii in DNA extracted from blood samples collected from 318 stray cats and dogs sampled from Deqing, Wenzhou, Yiwu, Lishui and Zhoushan cities across Zhejiang province, Eastern China. The LAMP-LFD device detected T. gondii DNA in 4.76 and 4.69% of stray cats and dogs, respectively. In conclusion, the developed LAMP-LFD assay is efficient, minimizes aerosol contamination, and is therefore suitable for detecting T. gondii across basic medical institutions and field settings.

Indexed as

CryptosporidiosisCryptosporidiumToxoplasmaAnimalsCatsChinaDogsMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesSensitivity and Specificity529-REDiagnosisLAMPLFDPrevalenceToxoplasma gondii

Identifiers

PMID33944774
PMCPMC8095094
OpenAlexW3159334187

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.