ArticleAmino acids2021
Novel antimicrobial cruzioseptin peptides extracted from the splendid leaf frog, Cruziohyla calcarifer.
Article in Amino acids, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 23 citations in OpenAlex.
- Domestic pig skin peptides: targeted starvation therapy for gram-positive bacteria MRSA through ABC transporters.BMC microbiology · 2026Article
- The Multifaceted Importance of Amphibians: Ecological, Biomedical, and Socio-Economic Perspectives.Biology · 2026Review
- Exploring the mechanisms of action of the antimicrobial peptide CZS-5 against Trypanosoma cruzi epimastigotes: insights from metabolomics and molecular dynamics.Parasites & vectors · 2025Article
- Identification, Synthesis, and In Vitro Activities of Antimicrobial Peptide from African Catfish against the Extended-Spectrum Beta-Lactamase (ESBL)-ProducingPharmaceutics · 2024Article
- Applications of tandem mass spectrometry (MS/MS) in antimicrobial peptides field: Current state and new applications.Heliyon · 2024Review
- Computational modelling of the antimicrobial peptides Cruzioseptin-4 extracted from the frog Cruziohyla calcarifer and Pictuseptin-1 extracted from the frog Boana picturata.Scientific reports · 2024Article
- Using the local symmetry in amino acids sequences of polypeptides to improve the predictive potential of models of their inhibitor activity.Amino acids · 2023Article
- Dual antibacterial mechanism of [K4K15]CZS-1 againstFrontiers in microbiology · 2023Article
- Antimicrobial peptides: Defending the mucosal epithelial barrier.Frontiers in oral health · 2022Review
- ADMET profiling and molecular docking of potential antimicrobial peptides previously isolated from African catfish,Frontiers in molecular biosciences · 2022Article
- Identification of Antimicrobial Peptides Isolated From the Skin Mucus of African Catfish,Frontiers in microbiology · 2021Article
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
Antimicrobial peptides (AMPs) constitute part of a broad range of bioactive compounds present on diverse organisms, including frogs. Peptides, produced in the granular glands of amphibian skin, constitute a component of their innate immune response, providing protection against pathogenic microorganisms. In this work, two novel cruzioseptins peptides, cruzioseptin-16 and -17, extracted from the splendid leaf frog Cruziohyla calcarifer are presented. These peptides were identified using molecular cloning and tandem mass spectrometry. Later, peptides were synthetized using solid-phase peptide synthesis, and their minimal inhibitory concentration and haemolytic activity were tested. Furthermore, these two cruzioseptins plus three previously reported (CZS-1, CZS-2, CZS-3) were computationally characterized. Results show that cruzioseptins are 21-23 residues long alpha helical cationic peptides, with antimicrobial activity against E. coli, S. aureus, and C. albicans and low haemolytic effect. Docking results agree with the principal action mechanism of cationic AMPs that goes through cell membrane disruption due to electrostatic interactions between cationic residues in the cruzioseptins and negative phosphate groups in the pathogen cell membrane. An action mechanism through enzymes inhibition was also tried, but no conclusive results about this mechanism were obtained.
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