Evidence map›Paper›PMID 33941076›Full record

ArticleBMC genomics2021

Genomic analysis of novel Yarrowia-like yeast symbionts associated with the carrion-feeding burying beetle Nicrophorus vespilloides.

Karina Brinkrolf, Shantanu P Shukla, Sven Griep, Oliver Rupp, Philipp Heise, Alexander Goesmann, David G Heckel, Heiko Vogel, Andreas Vilcinskas

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.8field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Karina BrinkrolfDepartment of Bioresources, Fraunhofer Institute for Molecular Biology and Applied Ecology, Ohlebergsweg 12, 35392, Giessen, Germany. karina.brinkrolf@bio.uni-giessen.de.
Shantanu P ShuklaDepartment of Entomology, Max Planck Institute for Chemical Ecology, Jena, Germany.
Sven GriepBioinformatics and Systems Biology, Justus Liebig University Giessen, Heinrich-Buff-Ring 58, 35302, Giessen, Germany.
Oliver RuppBioinformatics and Systems Biology, Justus Liebig University Giessen, Heinrich-Buff-Ring 58, 35302, Giessen, Germany.
Philipp HeiseDepartment of Bioresources, Fraunhofer Institute for Molecular Biology and Applied Ecology, Ohlebergsweg 12, 35392, Giessen, Germany.
Alexander GoesmannBioinformatics and Systems Biology, Justus Liebig University Giessen, Heinrich-Buff-Ring 58, 35302, Giessen, Germany.
David G HeckelDepartment of Entomology, Max Planck Institute for Chemical Ecology, Jena, Germany.
Heiko VogelDepartment of Entomology, Max Planck Institute for Chemical Ecology, Jena, Germany.
Andreas VilcinskasDepartment of Bioresources, Fraunhofer Institute for Molecular Biology and Applied Ecology, Ohlebergsweg 12, 35392, Giessen, Germany.
University of Giessen · DEMax Planck Institute for Chemical Ecology · DEFraunhofer Institute for Molecular Biology and Applied Ecology · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMutualistic interactions with microbes can help insects adapt to extreme environments and unusual diets. An intriguing example is the burying beetle Nicrophorus vespilloides, which feeds and reproduces on small vertebrate carcasses. Its fungal microbiome is dominated by yeasts that potentially facilitate carcass utilization by producing digestive enzymes, eliminating cadaver-associated toxic volatiles (that would otherwise attract competitors), and releasing antimicrobials to sanitize the microenvironment. Some of these yeasts are closely related to the biotechnologically important species Yarrowia lipolytica.

resultsTo investigate the roles of these Yarrowia-like yeast (YLY) strains in more detail, we selected five strains from two different phylogenetic clades for third-generation sequencing and genome analysis. The first clade, represented by strain B02, has a 20-Mb genome containing ~ 6400 predicted protein-coding genes. The second clade, represented by strain C11, has a 25-Mb genome containing ~ 6300 predicted protein-coding genes, and extensive intraspecific variability within the ITS-D1/D2 rDNA region commonly used for species assignments. Phenotypic microarray analysis revealed that both YLY strains were able to utilize a diverse range of carbon and nitrogen sources (including microbial metabolites associated with putrefaction), and can grow in environments with extreme pH and salt concentrations.

conclusionsThe genomic characterization of five yeast strains isolated from N. vespilloides resulted in the identification of strains potentially representing new YLY species. Given their abundance in the beetle hindgut, and dominant growth on beetle-prepared carcasses, the analysis of these strains has revealed the genetic basis of a potential symbiotic relationship between yeasts and burying beetles that facilitates carcass digestion and preservation.

Indexed as

ColeopteraYarrowiaAnimalsGenomicsPhylogenySymbiosisCarrion beetlesDetoxificationDigestionEphemeral resourcesMetabolic profilingNicrophorus vespilloidesrDNA variabilityTranscriptomicsYarrowia

Identifiers

PMID33941076
PMCPMC8091737
OpenAlexW3158652163

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.