Evidence map›Paper›PMID 33940022›Full record

ArticleBiophysical journal2021

The geometry of diphtheria toxoid CRM197 channel assessed by thiazolium salts and nonelectrolytes.

Oleg Ya Shatursky, Kyrylo Yu Manoilov, Oksana B Gorbatiuk, Mariya O Usenko, Dariia A Zhukova, Andriy I Vovk, Oleksandr L Kobzar, Irene O Trikash, Tatiana A Borisova, Denys V Kolibo and 1 more

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Article in Biophysical journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

11 authors.

Oleg Ya ShaturskyDepartment of Neurochemistry, Palladin Institute of Biochemistry, NAS of Ukraine, Leontovich Str. 9, Kyiv 01054, Ukraine. Electronic address: olegshatursky@biochem.kiev.ua.
Kyrylo Yu ManoilovDepartment of Molecular Immunology, Palladin Institute of Biochemistry, NAS of Ukraine, Leontovich Str. 9, Kyiv 01054, Ukraine.
Oksana B GorbatiukDepartment of Cell Regulatory Mechanisms, Institute of Molecular Biology and Genetics NAS of Ukraine, Zabolotnogo Str. 150, Kyiv 03143, Ukraine; State Institute of Genetic and Regenerative Medicine, NAMS of Ukraine, Andriivsky ds. 28 A, Kyiv, Ukraine.
Mariya O UsenkoDepartment of Cell Regulatory Mechanisms, Institute of Molecular Biology and Genetics NAS of Ukraine, Zabolotnogo Str. 150, Kyiv 03143, Ukraine; State Institute of Genetic and Regenerative Medicine, NAMS of Ukraine, Andriivsky ds. 28 A, Kyiv, Ukraine.
Dariia A ZhukovaDepartment of Molecular Immunology, Palladin Institute of Biochemistry, NAS of Ukraine, Leontovich Str. 9, Kyiv 01054, Ukraine.
Andriy I VovkDepartment of Bioorganic Mechanisms, V.P. Kukhar Institute of Bioorganic Chemistry and Petrochemistry, NAS of Ukraine, Murmanska Str. 1, Kyiv 02094, Ukraine.
Oleksandr L KobzarDepartment of Bioorganic Mechanisms, V.P. Kukhar Institute of Bioorganic Chemistry and Petrochemistry, NAS of Ukraine, Murmanska Str. 1, Kyiv 02094, Ukraine.
Irene O TrikashDepartment of Neurochemistry, Palladin Institute of Biochemistry, NAS of Ukraine, Leontovich Str. 9, Kyiv 01054, Ukraine.
Tatiana A BorisovaDepartment of Neurochemistry, Palladin Institute of Biochemistry, NAS of Ukraine, Leontovich Str. 9, Kyiv 01054, Ukraine.
Denys V KoliboDepartment of Molecular Immunology, Palladin Institute of Biochemistry, NAS of Ukraine, Leontovich Str. 9, Kyiv 01054, Ukraine.
Serhiy V KomisarenkoDepartment of Molecular Immunology, Palladin Institute of Biochemistry, NAS of Ukraine, Leontovich Str. 9, Kyiv 01054, Ukraine.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The geometry of the channel formed by nontoxic derivative of diphtheria toxin CRM197 in lipid bilayer was determined using the dependence of single-channel conductance upon the hydrodynamic radii of different nonelectrolytes. It was found that the cis entrance of CRM197 channel on the side of membrane to which the toxoid was added at pH 4.8 and the trans entrance on the opposite side at pH 6.0 had effective radii of 3.90 and 3.48 Å, respectively. The 3-alkyloxycarbonylmethyl-5-(2-hydroxyethyl)-4-methyl-1,3-thiazolium salts reversibly reduced current via CRM197 channels. The potency of the blockers increased with increasing length of alkyl chain at symmetric pH 6.0 and remained high and stable at pH 4.8 on the cis side. Comparative analysis of CRM197 and amphotericin B pore size with the inhibitory action of thiazolium salts revealed a significant increase in CRM197 pore dimension at pH 6.0. Addition of thiazolium salt with nine carbons alkyl tail increased by ∼30% the viability of human carcinoma cells A431 treated with diphtheria toxin.

Indexed as

Ion ChannelsSaltsBacterial ProteinsDiphtheria ToxoidHumansMembrane PotentialsBacterial ProteinsCRM197 (non-toxic variant of diphtheria toxin)Diphtheria ToxoidIon ChannelsSalts

Identifiers

PMID33940022
PMCPMC8390859

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