Evidence map›Paper›PMID 33939145›Full record

ArticleDigestive diseases and sciences2022

Correlation of Probe-Based Confocal Laser Endomicroscopy (pCLE) and Mucosal Integrity Testing (MIT) with Epithelial Barrier Function and Presence of Gastroesophageal Reflux Disease (GERD).

Pritesh R Mutha, Matthew Fasullo, Shijian Chu, Mitchell L Schubert, Alvin Zfass, Patricia Cooper, Mankanchan Gill, Le Kang, Michael Vaezi, Stu Wildhorn and 2 more

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Article in Digestive diseases and sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Pritesh R MuthaDivision of Gastroenterology, Hunter Holmes McGuire Richmond VA Medical Center, Gastroenterology # 111N, Richmond, VA, 23249, USA. Pritesh.Mutha@va.gov.ORCID 0000-0002-7749-6097
Matthew FasulloDivision of Gastroenterology, Virginia Commonwealth University, Richmond, VA, USA.
Shijian ChuDivision of Mathematics, General and Health Sciences, John Tyler Community College, Chester, VA, USA.
Mitchell L SchubertDivision of Gastroenterology, Hunter Holmes McGuire Richmond VA Medical Center, Gastroenterology # 111N, Richmond, VA, 23249, USA.
Alvin ZfassDivision of Gastroenterology, Hunter Holmes McGuire Richmond VA Medical Center, Gastroenterology # 111N, Richmond, VA, 23249, USA.
Patricia CooperDivision of Gastroenterology, Hunter Holmes McGuire Richmond VA Medical Center, Gastroenterology # 111N, Richmond, VA, 23249, USA.
Mankanchan GillDivision of Gastroenterology, Hunter Holmes McGuire Richmond VA Medical Center, Gastroenterology # 111N, Richmond, VA, 23249, USA.
Le KangDepartment of Biostatistics, Virginia Commonwealth University, Richmond, VA, USA.
Michael VaeziDepartment of Gastroenterology and Hepatology, Vanderbilt University, Nashville, TN, USA.
Stu WildhornDiversatek Healthcare, Highlands Ranch, CO, USA.
Chris SlaughterDepartment of Biostatistics, Vanderbilt University, Nashville, TN, USA.
Tilak ShahDivision of Gastroenterology, Hunter Holmes McGuire Richmond VA Medical Center, Gastroenterology # 111N, Richmond, VA, 23249, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionEpithelial barrier function (EBF) disruption is a key mechanism underlying gastroesophageal reflux disease (GERD). Our aim was to assess whether two novel technologies, probe-based confocal laser endomicroscopy (pCLE) and mucosal integrity testing (MIT), could assess EBF.

methodsWe prospectively enrolled patients undergoing upper endoscopy for refractory GERD or non-GERD conditions. Patients underwent esophagogastroduodenoscopy, pCLE, MIT, esophageal biopsy at 2 cm and 6 cm above the esophagogastric junction, and wireless pH testing. To assess EBF in vitro, biopsies were mounted in a mini-Ussing chamber, 1 ml of fluorescein was instilled on the mucosal side, and concentration of fluorescein on the serosal side was measured at 3 h.

resultsWe enrolled 54 subjects (28 GERD, 26 non-GERD based on Lyon consensus criteria). In vivo permeability assessed by pCLE did not differ significantly between GERD vs. non-GERD patients and did not correlate with in vitro permeability. Mean MIT at 2 cm was lower in GERD compared to non-GERD (1914 vs. 3727 ohms). MIT correlated inversely with in vitro permeability at 2 cm and at 6 cm. Using a predictive model that used slope and intercept of MIT at 2 cm and 6 cm, sensitivity and specificity of MIT at identifying GERD was 76% and 72%, respectively.

conclusionpCLE did not differentiate GERD vs non-GERD and did not correlate with EBF measured in vitro. MIT, on the other hand, may be more promising as it differentiated GERD vs non-GERD and correlated with EBF measured in vitro.

Indexed as

Gastroesophageal RefluxElectric ImpedanceEsophagoscopyFluoresceinsHumansLasersProspective StudiesFluoresceinsBarrett esophagusDiagnostic technique, Digestive systemElectric impedanceGastroesophageal refluxMicroscopy, Confocal

Identifiers

PMID33939145

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.