ArticleChembiochem : a European journal of chemical biology2021
Isopentenol Utilization Pathway for the Production of Linalool in Escherichia coli Using an Improved Bacterial Linalool/Nerolidol Synthase.
Article in Chembiochem : a European journal of chemical biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 48 citations in OpenAlex.
- Engineering an ATP-saving mevalonate pathway for high-efficiency S-(+)-linalool production inSynthetic and systems biotechnology · 2026Article
- Expanding the substrate scope of a bacterial monoterpene synthase for the production of sesquiterpenoid and diterpenoid products.The FEBS journal · 2026Article
- Unravelling the aromatic symphony: redirecting bifunctional mushroom synthases towards linalool monofunctionality.Advanced biotechnology · 2025Article
- Expanding catalytic promiscuity of a bifunctional terpene synthase through a single mutation-induced change in hydrogen-bond network within the catalytic pocket.Synthetic and systems biotechnology · 2024Article
- Two-Phase Fermentation Systems for Microbial Production of Plant-Derived Terpenes.Molecules (Basel, Switzerland) · 2024Review
- Decoding Catalysis by Terpene Synthases.ACS catalysis · 2023Review
- Systematic Mining and Evaluation of the Sesquiterpene Skeletons as High Energy Aviation Fuel Molecules.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Actinomycetes as Producers of Biologically Active Terpenoids: Current Trends and Patents.Pharmaceuticals (Basel, Switzerland) · 2023Review
- Structural Understanding of Fungal Terpene Synthases for the Formation of Linear or Cyclic Terpene Products.ACS catalysis · 2023Article
- Combinatorial Engineering of Upper Pathways and Carotenoid Cleavage Dioxygenase in Escherichia coli for Pseudoionone Production.Applied biochemistry and biotechnology · 2022Article
- Natural Compounds in the Battle against Microorganisms-Linalool.Molecules (Basel, Switzerland) · 2022Review
- Enhancement of linalool production in Saccharomyces cerevisiae by utilizing isopentenol utilization pathway.Microbial cell factories · 2022Article
- How a 10-ACS catalysis · 2022Article
- Optimization of microbial cell factories for astaxanthin production: Biosynthesis and regulations, engineering strategies and fermentation optimization strategies.Synthetic and systems biotechnology · 2022Review
- Molecular Determinants of Carbocation Cyclisation in Bacterial Monoterpene Synthases.Chembiochem : a European journal of chemical biology · 2022Article
- Predictive Engineering of Class I Terpene Synthases Using Experimental and Computational Approaches.Chembiochem : a European journal of chemical biology · 2022Review
- Alternative metabolic pathways and strategies to high-titre terpenoid production inNatural product reports · 2022Review
- Toward improved terpenoids biosynthesis: strategies to enhance the capabilities of cell factories.Bioresources and bioprocessing · 2022Review
- Bioproduction of Linalool From Paper Mill Waste.Frontiers in bioengineering and biotechnology · 2022Article
- Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Linalool is a monoterpenoid used as a fragrance ingredient, and is a promising source for alternative fuels. Synthetic biology offers attractive alternative production methods compared to extraction from natural sources and chemical synthesis. Linalool/nerolidol synthase (bLinS) from Streptomyces clavuligerus is a bifunctional enzyme, producing linalool as well as the sesquiterpenoid nerolidol when expressed in engineered Escherichia coli harbouring a precursor terpenoid pathway such as the mevalonate (MVA) pathway. Here we identified two residues important for substrate selection by bLinS, L72 and V214, where the introduction of bulkier residues results in variants with reduced nerolidol formation. Terpenoid production using canonical precursor pathways is usually limited by numerous and highly regulated enzymatic steps. Here we compared the canonical MVA pathway to the non-canonical isopentenol utilization (IU) pathway to produce linalool using the optimised bLinS variant. The IU pathway uses isoprenol and prenol to produce linalool in only five steps. Adjusting substrate, plasmid system, inducer concentration, and cell strain directs the flux towards monoterpenoids. Our integrated approach, combining enzyme engineering with flux control using the artificial IU pathway, resulted in high purity production of the commercially attractive monoterpenoid linalool, and will guide future efforts towards efficient optimisation of terpenoid production in engineered microbes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.