Evidence map›Paper›PMID 33937927›Full record

ArticleApplied microbiology and biotechnology2021

Ile258Met mutation of Brucella melitensis 7α-hydroxysteroid dehydrogenase significantly enhances catalytic efficiency, cofactor affinity, and thermostability.

Zhiyong Liu, Rongzhen Zhang, Wenchi Zhang, Yan Xu

Abstract read
PubMed Publisher
In one paragraph

Article in Applied microbiology and biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.1field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 16 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 2 countries.

Zhiyong LiuKey Laboratory of Industrial Biotechnology of Ministry of Education & School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, People's Republic of China.
Rongzhen ZhangKey Laboratory of Industrial Biotechnology of Ministry of Education & School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, People's Republic of China. rzzhang@jiangnan.edu.cn.ORCID http://orcid.org/0000-0001-6078-0174
Wenchi ZhangSolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA.
Yan XuKey Laboratory of Industrial Biotechnology of Ministry of Education & School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, People's Republic of China.
Jiangnan University · CNJohns Hopkins University · US

Funding

Postgraduate Research & Practice Innovation Program of Jiangsu Province 1012050205205974the National First-class Discipline Program of Light Industry Technology and Engineering LITE2018-12the National Key research and Development Program of China 2018YFA0900302the National Science Foundation of China 31970045the Program of Introducing Talents of Discipline to Universities 111-2-06Top-notch Academic Programs Project of Jiangsu Higher Education Institutions No
6 · The paper itself

Abstract

NAD(H)-dependent 7α-hydroxysteroid dehydrogenase catalyzes the oxidation of chenodeoxycholic acid to 7-oxolithocholic acid. Here, we designed mutations of Ile258 adjacent to the catalytic pocket of Brucella melitensis 7α-hydroxysteroid dehydrogenase. The I258M variant gave a 4.7-fold higher k

Indexed as

Brucella melitensisCatalysisHydroxysteroid DehydrogenasesKineticsMutation7 alpha-hydroxysteroid dehydrogenaseHydroxysteroid Dehydrogenases7α-Hydroxysteroid dehydrogenaseBrucella melitensisChenodeoxycholic acidChiral catalysisProtein engineering

Identifiers

PMID33937927
OpenAlexW3158846206

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.